Effect on separation of injecting samples in a solvent different from the mobile phase
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Updated: Jul 16, 2026

High-throughput and Comprehensive Drug Surveillance Using Multisegment Injection-Capillary Electrophoresis-Mass Spectrometry
Published on: April 23, 2019
Knut Gedicke1, Dorota Antos, Andreas Seidel-Morgenstern
1Chemical Process Engineering, Otto-von-Guericke-University, Universitätsplatz 2, 39106 Magdeburg, Germany. knut.gedicke@vst.uni-magdeburg.de
Abstract:
The separation of compounds possessing low solubility in the mobile phase could be improved by applying stronger solvents for dissolving the feed. A model system has been investigated with ethanol-water as the mobile phase, Chirobiotic T as the stationary phase and D,L-threonine as the sample dissolved in pure water. The adsorption isotherms of D,L-threonine were determined as a function of the water content by means of a peak fitting method. Optimal injection conditions of elution chromatography were determined by process simulation. Finally, the optima obtained were verified with experimental data.
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