Related Experiment Video
Updated: Jul 16, 2026
Screening and Identification of Small Peptides Targeting Fibroblast Growth Factor Receptor2 using a Phage Display Peptide Library
Published on: September 30, 2019
Panning and identification of a colon tumor binding peptide from a phage display peptide library
Yangde Zhang1, Jiji Chen, Yanqiong Zhang
1National Key Laboratory of Nanobiological Technology, Changsha, Hunan, China.
Abstract:
Tumor-targeting therapy can be an efficacious way to cure a malignant tumor in clinical trials. Phage display is a molecular diversity technology that allows the presentation of a large number of peptides or proteins on the surface of filamentous phage for various applications. In this study, we report on using phage display to generate peptide libraries that bind to colon cancer tissues. To accomplish this, we developed a screening protocol that contained 3 rounds of in vitro positive panning on colon cancer cells (SW480) and 2 rounds of subtractive screening in vitro on normal human intestinal epithelial cells with a phage display-7 peptide library. After several rounds of panning, both phage titer and recovery efficiency were significantly improved. Through a cell-based enzyme-linked immunosorbent assay, immunofluorescence, in vivo binding assay, immunocytochemical staining, and immunohistochemical staining, peptide CP15 (VHLGYAT) was demonstrated to be the most effective peptide in targeting tumor cells (SW480 and HT29 cells) and tumor tissues but not the normal human intestinal epithelial cells and control colon tissue. These studies suggest that peptide CP15 may be a promising lead candidate in the development of a useful colon tumor diagnostic and targeted drug delivery agent.
Insights
Researchers developed a phage display method to identify peptides targeting colon cancer. Peptide CP15 effectively targets colon tumor cells and tissues, showing potential for diagnostics and drug delivery.
Area of Science:
- Biotechnology
- Molecular Biology
- Oncology
Background:
- Tumor-targeting therapy offers a promising strategy for treating malignant tumors.
- Phage display is a powerful technology for generating diverse peptide libraries for various applications.
Purpose of the Study:
- To utilize phage display to generate and identify peptide binders specific to colon cancer tissues.
- To evaluate the efficacy of identified peptides in targeting colon cancer cells and tissues.
Main Methods:
- A phage display-7 peptide library was screened using a multi-round protocol involving positive panning on colon cancer cells (SW480) and subtractive screening on normal intestinal cells.
- Screening involved 3 rounds of in vitro positive panning and 2 rounds of in vitro subtractive screening.
- Candidate peptide efficacy was assessed using cell-based ELISA, immunofluorescence, in vivo binding assays, and immunohistochemical staining.
Main Results:
- Phage titer and recovery efficiency significantly improved after multiple screening rounds.
- Peptide CP15 (VHLGYAT) demonstrated superior binding to colon cancer cells (SW480, HT29) and tumor tissues.
- CP15 showed no significant binding to normal human intestinal epithelial cells or control colon tissue.
Conclusions:
- Peptide CP15 is a highly specific and effective colon tumor-targeting peptide.
- CP15 holds potential as a lead candidate for developing colon cancer diagnostic agents and targeted drug delivery systems.
More Related Videos
08:31Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
Published on: December 1, 2020
13:49Semi-automated Biopanning of Bacterial Display Libraries for Peptide Affinity Reagent Discovery and Analysis of Resulting Isolates
Published on: December 6, 2017