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Published on: March 12, 2021
Expression profiling of peroxisome proliferator-activated receptor-delta (PPAR-delta) in mouse tissues using tissue
Hiroyuki Higashiyama1, Andrew N Billin, Yuji Okamoto
1Pharmacology Department, Tsukuba Research Laboratories and Nuclear Receptor Discovery Research, High Throughput Biology, Discovery Research, GlaxoSmithKline, 43 Wadai, 300-4247, Tsukuba, Ibaraki, Japan.
Peroxisome proliferator-activated receptor-delta (PPAR-delta) is a transcription factor found throughout adult mouse tissues. This study mapped PPAR-delta distribution, revealing its widespread presence and nuclear localization, suggesting diverse physiological roles.
Area of Science:
- Molecular Biology
- Physiology
- Immunology
Background:
- Peroxisome proliferator-activated receptor-delta (PPAR-delta) regulates fatty acid oxidation and mitochondrial biogenesis.
- Its systemic physiological functions require comprehensive tissue distribution analysis.
Purpose of the Study:
- To elucidate the systemic physiological functions of PPAR-delta.
- To examine the tissue distribution and localization of PPAR-delta in adult mouse tissues.
Main Methods:
- Tissue microarray (TMA)-based immunohistochemistry was employed.
- High-throughput protein localization and distribution profiling was performed.
Main Results:
- PPAR-delta positive signals were detected across multiple organ systems, including cardiovascular, urinary, respiratory, digestive, endocrine, nervous, hematopoietic, immune, musculoskeletal, sensory, and reproductive systems.
- PPAR-delta immunoreactivity was predominantly nuclear, with some cytoplasmic localization observed in specific cell types like neurons and islet cells.
Conclusions:
- The widespread expression profiling of PPAR-delta suggests diverse physiological roles in multiple organ systems.
- TMA-based immunohistochemistry is an effective high-throughput method for assessing protein distribution.