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Updated: Jul 16, 2026

Impact Assessment of Repeated Exposure of Organotypic 3D Bronchial and Nasal Tissue Culture Models to Whole Cigarette Smoke
Published on: February 12, 2015
[Second-hand smoke exposure in Florence and Belluno before and after the Italian smoke-free legislation]
Antonio Gasparrini1, Giuseppe Gorini, Daniela Marcolina
1UO Epidemiologia ambientale occupazionale, CSPO, Firenze. a.gasparrini@cspo.it
Introduction:
a law banning smoking in enclosed public places entered into force in Italy on January 10th 2005.
Objective:
to compare the SHS exposure before and after the coming into force of the new anti-smoking law, with direct measurements in public venues and workplaces.
Methods:
vapour-phase nicotine was measured using passive samplers, with personal and environmental sampling.
Setting:
samples were collected in 10 municipality offices, 10 industry buildings and 11 public venues (4 discos and 7 pubs) in two towns (Florence and Belluno) from november 2004 to march 2005.
Results:
during the pre-ban period a wide range of nicotine concentrations was observed. Offices and industry sector exhibited very low concentrations, 0,47 e 0,40 microg/m3 in median, respectively. Highest concentrations were measured in pubs and discos (35,59 and 127,16 microg/m3). After the smoking ban, a noteworthy reduction in nicotine concentrations was found with a stronger effect in pubs and discos (95% of decrease).
Discussion:
the introduction of a national smoking ban led to a clear reduction in SHS exposure, with stronger results in environments less protected by previous regulations.
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