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Related Experiment Videos

Methods for measuring plasma hemoglobin in micromolar concentration compared.

V F Fairbanks1, S C Ziesmer, P C O'Brien

  • 1Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, MN 55905.

Clinical Chemistry
|January 1, 1992
PubMed
Summary

Accurate plasma hemoglobin measurement is crucial. Eight methods were tested, with the 578 nm absorbance assay showing precision and reliability, even with common interferences like icterus or lipemia.

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Area of Science:

  • Clinical Chemistry
  • Biomedical Analysis

Background:

  • Accurate quantification of plasma hemoglobin is essential for diagnosing and monitoring various medical conditions.
  • Existing measurement methods may be susceptible to interference from common biological substances.

Purpose of the Study:

  • To evaluate the performance of eight distinct methods for measuring plasma hemoglobin concentration.
  • To identify reliable methods for plasma hemoglobin assay, particularly in the presence of interfering substances.

Main Methods:

  • Assessed linearity, reproducibility, and concurrence across eight plasma hemoglobin measurement techniques.
  • Investigated the impact of icteric, lipemic, methemoglobin, and methemalbumin conditions on assay accuracy.
  • Utilized absorbance measurement at 578 nm with an Allen correction for precise oxyhemoglobin quantification.

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Main Results:

  • All methods except those affected by icterus, lipemia, methemoglobin, or methemalbumin showed acceptable performance.
  • The 578 nm absorbance method with Allen correction accurately measured oxyhemoglobin down to 0.01 g/L, unaffected by hyperlipidemia or hyperbilirubinemia.
  • Discrepancies between methods were noted in 11.6% of nonicteric patient samples; spectral examination aids in resolving these.

Conclusions:

  • The 578 nm absorbance method with Allen correction is a precise and reliable technique for measuring plasma oxyhemoglobin.
  • Interference from methemalbumin or methemoglobin requires specific detection methods beyond simple oxyhemoglobin measurement.
  • Method selection is critical, especially when dealing with complex biological matrices or potential interfering substances.