Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Heat stress attenuates ATP-depletion and pH-decrease during cardioplegic arrest.

Sebastian Vogt1, Dirk Troitzsch, Hashim Abdul-Khaliq

  • 1Biomedical Research Center, Cardiovascular Research Lab and Heart Surgery, University Hospital, Philipps-University Marburg, Marburg, Germany. vogts@med.uni-marburg.de

The Journal of Surgical Research
|March 6, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Assessing the contribution of rare variants to congenital heart disease through a large-scale case-control exome study.

NPJ genomic medicine·2026
Same author

Inhibitory Effect of ATP on Cytochrome c Oxidase Depends on Electron Entry Pathways by TCA Cycle Metabolites.

Cells·2026
Same author

Lowered Maternal and Paternal Plasma Concentrations of Choline Are Associated with the Severity of Congenital Heart Defects in the Offspring.

Nutrients·2026
Same author

Genetic alterations in poor-quality individually selected sperm highlight candidate biomarkers for male subfertility.

Scientific reports·2026
Same author

Malposition of a Port Catheter in the Azygos Vein: Endovascular Repositioning Using a Long Loop Snare Technique.

Catheterization and cardiovascular interventions : official journal of the Society for Cardiac Angiography & Interventions·2026
Same author

Add-Ons of Heart Disease from the Cardiosurgical Perspective: Gender, Blood Groups and Renal Function.

Medical sciences (Basel, Switzerland)·2026
Same journal

Survival Following Neoadjuvant Chemoradiation in Rectosigmoid Junction Adenocarcinoma: SEER Analysis.

The Journal of surgical research·2026
Same journal

Operative Case Trends in General Surgery Residency: Growth Across All Levels.

The Journal of surgical research·2026
Same journal

The Plantaris Tendon in Surgery: A Scoping Review of Reconstructive Roles Beyond Orthopedics.

The Journal of surgical research·2026
Same journal

Open Versus Minimally Invasive Surgery for Congenital Diaphragmatic Hernia Repair: A Systematic Review and Meta-analysis.

The Journal of surgical research·2026
Same journal

Time-resolved Impact of Smoke Evacuation on Particulate Exposure During Laparoscopic Cholecystectomy.

The Journal of surgical research·2026
Same journal

Geriatric Age is an Independent Risk Factor for Worse Clinical Outcomes After Motorcycle Collision.

The Journal of surgical research·2026
See all related articles

Heat stress pretreatment improves heart function and energy metabolism after prolonged cold ischemia by inducing heat shock proteins (HSPs). This protective effect preserves high-energy phosphates and attenuates pH decrease during ischemia and reperfusion.

Area of Science:

  • Cardiology
  • Biochemistry
  • Physiology

Background:

  • Heat stress induction is known to enhance myocardial tolerance against ischemia.
  • This study investigates cardiac energy metabolism following heat shock protein (HSP) induction in neonatal rabbit hearts subjected to prolonged cold cardioplegic ischemia.

Purpose of the Study:

  • To evaluate the impact of heat stress-induced HSP expression on cardiac energy metabolism and functional recovery after prolonged cold cardioplegic ischemia.
  • To assess the preservation of high-energy phosphates and myocardial pH during ischemia and reperfusion.

Main Methods:

  • Neonatal rabbit hearts were subjected to 2-hour cold cardioplegic ischemia.
  • Heat shock was induced by raising rectal temperature to 42.0-42.5°C for 15 minutes prior to ischemia.

Related Experiment Videos

  • Left ventricular function recovery was measured using aortic flow, cardiac output, and max dP/dt.
  • High-energy phosphate levels were analyzed using 31P-nuclear magnetic resonance spectroscopy.

    • Main Results:

    • Heat stress pretreatment led to significant improvements in left ventricular function (aortic flow, cardiac output, max dP/dt) post-reperfusion compared to controls (P < 0.05).
    • Myocardial energy metabolism was better preserved in heat-stressed hearts, evidenced by increased gamma-ATP, alpha-ATP, beta-ATP, and phosphocreatine levels.
    • The reduction in myocardial pH during ischemia was attenuated in the heat-stressed group.

    Conclusions:

    • Heat stress pretreatment confers beneficial effects on functional myocardial recovery and energy metabolism following prolonged cold cardioplegic ischemia.
    • The observed protective effects suggest a role for heat shock proteins in preserving mitochondrial membrane potential and attenuating ischemic damage.