Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...
Methods of Classification and Identification01:28

Methods of Classification and Identification

Bacterial identification relies on a diverse array of techniques to classify and understand microorganisms, each tailored to uncover specific characteristics. Traditional morphological approaches, while still valuable, are limited for closely related or structurally simple organisms. Modern methods integrate biochemical, serological, genetic, and advanced molecular tools to achieve greater accuracy.Morphological and Biochemical TechniquesMorphological characteristics, such as cell shape and...
Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
Modern Molecular Taxonomy01:29

Modern Molecular Taxonomy

Advancements in molecular biology have revolutionized the identification and characterization of bacteria, with multiple methods leveraging DNA sequencing for enhanced precision. As sequencing technologies improve and costs decline, these approaches are increasingly used in clinical, environmental, and evolutionary studies.Multilocus Sequence Typing (MLST) examines several housekeeping genes, essential chromosomal genes encoding cellular functions, to distinguish strains. Approximately...
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a visible...
Applications of Molecular Taxonomy01:20

Applications of Molecular Taxonomy

Molecular taxonomy has revolutionized the understanding and classification of bacteria, providing precise insights into their diversity, evolutionary relationships, and ecological roles. By utilizing molecular techniques such as DNA sequencing and fingerprinting, researchers have made significant strides in various fields related to bacterial studies.Resolving Taxonomic AmbiguitiesMolecular taxonomy has been instrumental in distinguishing closely related bacterial species initially thought to...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reactivation of Coccidioidomycosis in a Mouse Model of Asymptomatic Controlled Disease.

Journal of fungi (Basel, Switzerland)·2022
Same author

Cytologic appearance of hibernoma in two dogs.

Veterinary clinical pathology·2020
Same author

Letter to the Editor Regarding "Interstitial Adipocytes in the Beagle Dog and New Zealand White Rabbit Choroid Plexus".

Toxicologic pathology·2019
Same author

Retrospective analysis of cutaneous lesions in 23 canine and 17 feline cases of coccidioidomycosis seen in Arizona, USA (2009-2015).

Veterinary dermatology·2016
Same author

Investigations of Salmonella enterica serovar newport infections of oysters by using immunohistochemistry and knockout mutagenesis.

Applied and environmental microbiology·2012
Same author

Guidelines for resident training in veterinary clinical pathology. III: cytopathology and surgical pathology.

Veterinary clinical pathology·2009

Related Experiment Video

Updated: Jul 16, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Fungal diagnostics: current techniques and future trends.

Sharon M Dial1

  • 1Department of Veterinary Science and Microbiology, Arizona Veterinary Diagnostic Laboratory, University of Arizona, 2831 North Freeway, Tucson, AZ 85705, USA. sdial@u.arizona.edu

The Veterinary Clinics of North America. Small Animal Practice
|March 6, 2007
PubMed
Summary

Diagnosing fungal diseases requires careful review of patient history, clinical signs, and laboratory results. A comprehensive approach integrating various diagnostic methods is crucial for accurate identification of fungal agents.

Related Experiment Videos

Last Updated: Jul 16, 2026

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level
08:19

Microfluidic Tools for Probing Fungal-Microbial Interactions at the Cellular Level

Published on: June 23, 2022

Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Diagnostic Pathology

Background:

  • Fungal infections present diagnostic challenges due to their varied clinical manifestations.
  • Fungal diseases can mimic other serious conditions like bacterial infections and cancers.
  • Clinicians must be aware of endemic and emerging fungal pathogens.

Purpose of the Study:

  • To outline a comprehensive diagnostic strategy for fungal diseases.
  • To emphasize the importance of integrating diverse diagnostic modalities.
  • To improve the accuracy of identifying causative fungal agents.

Main Methods:

  • Correlating clinical presentation and physical examination findings.
  • Utilizing clinical pathology, including histopathology.
  • Incorporating serology, fungal cultures, immunohistochemistry, and molecular techniques.

Main Results:

  • A multi-faceted diagnostic approach enhances the identification of fungal pathogens.
  • Integration of clinical and laboratory data is key to overcoming diagnostic hurdles.
  • Awareness of geographic distribution of fungal diseases is essential.

Conclusions:

  • A holistic diagnostic strategy is optimal for identifying fungal diseases.
  • Combining traditional and advanced techniques improves diagnostic yield.
  • Early and accurate diagnosis is critical for effective patient management.