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Decrease in glutathione (GSH) content in bovine sperm after cryopreservation: comparison between two extenders.

G Stradaioli1, T Noro, L Sylla

  • 1Dipartimento di Scienze Animali, Università di Udine, via delle Scienze 208, 33100 Udine, Italy. giuseppe.stradaioli@uniud.it

Theriogenology
|March 6, 2007
PubMed
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Commercial extenders better preserve bull semen quality after cryopreservation by maintaining higher glutathione (GSH) levels, reducing oxidative stress and improving sperm viability and function.

Area of Science:

  • Reproductive Biology
  • Biochemistry
  • Animal Science

Background:

  • Cryopreservation of semen can lead to oxidative stress and reduced antioxidant levels, negatively impacting sperm quality.
  • Glutathione (GSH) is a key intracellular antioxidant depleted during semen cryopreservation.

Purpose of the Study:

  • To evaluate the impact of two different semen extenders on bovine sperm quality after freezing and thawing.
  • To compare the efficacy of a commercial extender (Bioxcell) versus a traditional egg yolk tris-citrate extender in preserving sperm parameters and GSH levels.

Main Methods:

  • Bovine semen samples from five bulls were cryopreserved using either egg yolk tris-citrate or Bioxcell extenders.
  • Post-thaw analysis included assessment of sperm motility, capacitation status, acrosome reaction, and glutathione (GSH) levels.

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Main Results:

  • Bioxcell extender significantly preserved higher GSH content compared to egg yolk tris-citrate extender.
  • Spermatozoa frozen in Bioxcell exhibited improved motility, a higher proportion of non-capacitated sperm, and a lower incidence of acrosome reaction post-thaw.
  • The superior preservation by Bioxcell may be attributed to its higher initial GSH content, mitigating oxidative damage.

Conclusions:

  • Commercial Bioxcell extender demonstrates superior cryoprotective properties for bovine spermatozoa compared to egg yolk tris-citrate.
  • Maintaining adequate GSH levels in extenders is crucial for alleviating oxidative stress and enhancing sperm survival during cryopreservation.
  • Further in vivo studies are warranted to confirm these findings.