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Updated: Jul 16, 2026

A Novel Clinical Grade Isolation Method for Human Kidney Perivascular Stromal Cells
Published on: August 7, 2017
Neural cell adhesion molecule expression on renal interstitial cells.
Jasmina Marković-Lipkovski1, Claudia A Müller, Gerd Klein
1Institute of Pathology, University of Belgrade, 11000 Belgrade, Dr Subotića 1, Serbia. acal@matf.bg.ac.yu
Neural cell adhesion molecule (NCAM) is found on rare interstitial cells in adult kidneys, potentially representing renal progenitors. These NCAM+ cells may participate in early interstitial fibrosis.
Area of Science:
- Nephrology
- Cell Biology
- Developmental Biology
Background:
- Neural cell adhesion molecule (NCAM) is highly expressed during early kidney development.
- NCAM expression diminishes as the fetal kidney matures.
- NCAM in adult kidneys was previously thought to be restricted to nerves.
Purpose of the Study:
- To investigate NCAM expression in adult human kidneys.
- To characterize NCAM-expressing cells in healthy and diseased kidneys.
- To explore the role of NCAM+ cells in renal interstitial fibrosis.
Main Methods:
- Immunohistochemistry and western blot analysis of human kidney tissues.
- Double immunofluorescent staining for cell characterization.
- Analysis of NCAM expression in healthy and fibrotic kidneys.
Main Results:
- NCAM is expressed by rare, neurofilament-negative interstitial cells in adult kidneys.
- These cells co-express hematopoietic stem cell markers CD34 and CD133.
- Increased NCAM+ cells and the 140 kDa NCAM isoform were observed in early interstitial fibrosis.
Conclusions:
- A rare subpopulation of NCAM+ interstitial cells may represent renal progenitors.
- NCAM+ interstitial cells are implicated in the initial stages of interstitial fibrosis.
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