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Refined medium for direct isolation of Vibrio vulnificus from oyster tissue and seawater

Elizabeth Warner1, James D Oliver

  • 1Department of Biology, University of North Carolina at Charlotte, Charlotte, NC 28223, USA.

Applied and Environmental Microbiology
|March 6, 2007
PubMed

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...