Related Experiment Videos
Walking, cloning, and mapping with yeast artificial chromosomes: a contig encompassing D21S13 and D21S16
R Butler1, D J Ogilvie, P Elvin
1Biotechnology Department, ICI Pharmaceuticals, Macclesfield, Cheshire, United Kingdom.
Genomics
|January 1, 1992
Abstract:
Chromosome 21 has often been used as a model system for the development of genome mapping and cloning strategies in humans. In this report methods for systematic chromosome walking, cloning, and mapping are exemplified in the construction of a 1.5-Mb yeast artificial chromosome (YAC) contig encompassing and extending 400 kb beyond each of the genetic loci D21S13 and D21S16. Isolation of insert-terminal sequences from YACs in this contig provides a set of closely spaced physical markers. These have been used to generate a long-range genomic restriction map.