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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis
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Laser-capture Microdissection of Human Prostatic Epithelium for RNA Analysis

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A highly conserved protein secreted by the prostate cancer cell line PC-3 is expressed in benign and malignant

Camilla Valtonen-André1, Anders Bjartell, Rebecka Hellsten

  • 1Lund University, Division of Clinical Chemistry, Department of Laboratory Medicine, University Hospital MAS, S-205 02 Malmö, Sweden.

Biological Chemistry
|March 7, 2007
PubMed

Insights

Researchers identified a new gene on human chromosome 9, encoding PC3-secreted microprotein (PSMP). This protein is highly conserved and shows significance in prostate cancer development and detection.

Area of Science:

  • Genetics
  • Molecular Biology
  • Oncology

Background:

  • A novel gene on human chromosome 9 was identified.
  • Its translation product, PC3-secreted microprotein (PSMP), was characterized.
  • PSMP is homologous to beta-microseminoprotein (MSP), a prostate-secreted protein of unknown function.

Purpose of the Study:

  • To characterize the novel gene and its protein product, PSMP.
  • To investigate the role and significance of PSMP in prostate cancer.

Main Methods:

  • Gene sequencing and protein analysis.
  • Homology and conservation studies.
  • Expression analysis in cell lines and xenografts.
  • Immunohistochemical staining of human prostate cancer specimens.

Main Results:

  • PSMP gene has three exons encoding a 139-amino acid protein with a signal peptide.
  • PSMP is conserved across species, unlike MSP.
  • High PSMP transcript levels were found in PC-3 prostate cancer cells.
  • PSMP undergoes post-translational modification.
  • PSMP protein and mRNA were detected in xenograft tumors and human prostate tissues.

Conclusions:

  • PSMP is a conserved protein with potential significance in prostate cancer.
  • PSMP may serve as a biomarker or therapeutic target in prostate cancer.

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