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Related Experiment Videos

Disabled-2 is an epithelial surface positioning gene.

Dong-Hua Yang1, Kathy Q Cai, Isabelle H Roland

  • 1Ovarian Cancer and Tumor Cell Biology Programs, Department of Medical Oncology, Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111, USA.

The Journal of Biological Chemistry
|March 7, 2007
PubMed
Summary
This summary is machine-generated.

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Disabled-2 (Dab2) is crucial for primitive endoderm formation in mammalian embryos. Loss of Dab2 disrupts cell surface positioning and epithelial morphogenesis by impairing protein polarity.

Area of Science:

  • Developmental biology
  • Cell biology
  • Molecular genetics

Background:

  • Primitive endoderm formation is an early epithelial morphogenesis event in mammalian embryos.
  • Disabled-2 (Dab2) is known to mediate endocytic trafficking of clathrin-coated vesicles.
  • Dab2 deficiency in mouse embryos leads to defective primitive endoderm positioning and morphogenesis.

Purpose of the Study:

  • To investigate the role of Dab2 in epithelial cell surface positioning and polarized protein distribution.
  • To elucidate the mechanism by which Dab2 regulates primitive endoderm formation.
  • To identify Dab2 as a surface positioning gene.

Main Methods:

  • Analysis of mouse embryos and embryonic stem cells deficient in Dab2.
  • Investigation of cell surface protein localization (megalin, E-cadherin) in Dab2-deficient cells.

Related Experiment Videos

  • Assessment of cell positioning and epithelial layer formation in embryoid bodies.

    • Main Results:

    • Dab2-deficient cells fail to position correctly on the surface of cell aggregates.
    • Loss of Dab2 leads to defective primitive endoderm layer formation in embryoid bodies.
    • Dab2 mediates directional trafficking and polarized distribution of cell surface proteins like megalin and E-cadherin.

    Conclusions:

    • Loss of polarized distribution of cell surface proteins is the mechanism underlying defective positioning in Dab2-deficient embryos.
    • Dab2 functions as a surface positioning gene, regulating epithelial cell surface targeting.
    • Dab2 is essential for proper epithelial morphogenesis during early embryonic development.