Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Virus-derived platforms for visualizing protein associations inside cells.

Cathy L Miller1, Michelle M Arnold, Teresa J Broering

  • 1Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA. clm@iastate.edu

Molecular & Cellular Proteomics : MCP
|March 7, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Toward Realistic Autonomous Driving Dataset Augmentation: A Real-Virtual Fusion Approach with Inconsistency Mitigation.

Sensors (Basel, Switzerland)·2026
Same author

Comparative analysis of NSP5/VP2-induced viroplasm-like structures in rotavirus species A to J.

Journal of virology·2025
Same author

High-performance biodegradable poly(lactic acid) composites with xylan and lignin copolymer.

International journal of biological macromolecules·2025
Same author

The influence of the cytoskeleton on the development and behavior of viral factories in mammalian orthoreovirus.

Virology·2025
Same author

Reconstruction of biorefinery lignin into nanoparticles with controlled morphology and structure.

International journal of biological macromolecules·2024
Same author

Differential Drug Susceptibility across Trichomonasvirus Species Allows for Generation of Varied Isogenic Clones of <i>Trichomonas vaginalis</i>.

Pathogens (Basel, Switzerland)·2024

A new method uses orthoreovirus protein muNS to visualize protein-protein interactions within cells, identifying novel protein associations and complexes. This technique is simple, adaptable, and aids in understanding cellular functions.

Area of Science:

  • Cell Biology
  • Virology
  • Molecular Biology

Background:

  • Protein-protein interactions are fundamental to cellular processes.
  • Visualizing these interactions in living cells is crucial for understanding cell function.
  • Existing methods can be complex or lack throughput.

Purpose of the Study:

  • To develop a novel, simplified method for visualizing protein-protein associations within cells.
  • To demonstrate the utility of this method in identifying new protein interactions and complexes.
  • To leverage orthoreovirus protein muNS for creating cellular inclusions that recruit interacting proteins.

Main Methods:

  • Utilized orthoreovirus protein muNS to form cytoplasmic inclusions for protein recruitment.
  • Employed fluorescently tagged muNS fusion proteins to present other proteins (e.g., p53).

Related Experiment Videos

  • Applied light microscopy and three-color microscopy to visualize and analyze protein associations and complexes.

    • Main Results:

    • Identified a sixth orthoreovirus protein, RNA-dependent RNA polymerase lambda3, recruited by muNS.
    • Demonstrated recruitment of simian virus 40 large T antigen by a muNS-p53 fusion, revealing key interaction regions.
    • Confirmed p53 oligomerization and association with CREB-binding proteins within inclusions.
    • Identified a ternary complex of p53, simian virus 40 large T antigen, and retinoblastoma protein.

    Conclusions:

    • The developed method provides a simple and adaptable platform for studying protein-protein interactions in cells.
    • This technique facilitates the identification of novel protein associations and the characterization of protein complexes.
    • The findings highlight the potential of using viral proteins as tools in cell biology research.