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Monitoring Electroporation-Induced Changes in Action Potential Generation in Genetically Engineered Tet-On Spiking HEK cells

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Electric fields around and within single cells during electroporation-a model study.

Brian J Mossop¹, Roger C Barr, Joshua W Henshaw

¹Department of Biomedical Engineering, Duke University, 136 Hudson Hall, Box 90281, Durham, NC 27708, USA.

Annals of Biomedical Engineering

|March 7, 2007

Summary

Electroporation significantly alters intracellular electric fields, impacting molecular delivery. Simulations show large intracellular current variations, unlike minor extracellular changes, guiding targeted delivery strategies.

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Area of Science:

Biophysics
Cell Biology
Electroporation

Background:

Electric field-mediated molecular delivery relies on understanding intracellular electric field dynamics during electroporation.
Electroporation creates transient pores in cell membranes, altering electrical properties.

Purpose of the Study:

To simulate and analyze the electric field distribution within and around spherical cells during electroporation.
To quantify the impact of electroporation on intracellular vs. extracellular currents and fields.

Main Methods:

Macroscopic modeling of electroporation, treating the electroporated membrane as having reduced resistivity.
Simulations varied the size of the electroporated membrane region from zero to complete cell surface coverage.

Main Results:

Intracellular current varied by several orders of magnitude, while extracellular and total currents showed minimal change (<8% and <4%).
Electric fields near the cell center varied significantly, contrasting with fields across the permeabilized membrane.
Extracellular field redirection was localized near pores; intracellular fields were directed radially from pores.

Conclusions:

Electroporation causes substantial intracellular field modifications, crucial for molecular delivery.
Localized field redirection suggests passive uptake is pore-specific.
Radial intracellular fields may enhance the distribution of charged molecules within the cell.