Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Techniques for Isolation of Pure Cultures

Techniques for Isolation of Pure Cultures

Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Exploratory analysis of risk factors associated with antimicrobial resistance in urinary Escherichia coli from dogs in the United States (2018-2024).

BMC veterinary research·2026

Same author

Core-genome multilocus sequence types of the IncI1 plasmids identified in <i>Salmonella enterica</i> subsp. <i>Enterica</i> serovars Heidelberg and Kentucky from Canadian poultry production are associated with factors related to production, year, and geographical region.

Canadian journal of microbiology·2026

Same author

Knowledge, Attitudes and Practices Related to Antimicrobial Use and Resistance Among Livestock Sector Stakeholders in Seven Former Soviet Countries: A Multi-Country Regional Analysis.

Antibiotics (Basel, Switzerland)·2026

Same author

Trends in Necrotic Enteritis and Coccidiosis Control Practices in Canadian Poultry Flocks, 2018-2023.

Avian diseases·2026

Same author

Utilization Patterns and Implementation Barriers in Adoption of Teledentistry Within Romanian Dental Practice.

Healthcare (Basel, Switzerland)·2025

Same author

Insights into hepatitis B vaccination decision-making: Exploring vaccine preferences, health disparities, and practice patterns among patients and healthcare providers.

Human vaccines & immunotherapeutics·2025

Search research articles

Related Experiment Video

Updated: Jul 16, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Novel Campylobacter isolation method using hydrophobic grid membrane filter and semisolid medium.

Alfonso Valdivieso-Garcia¹, Kathleen Harris, Edward Riche

¹Laboratory for Foodborne Zoonoses, Public Health Agency of Canada, 110 Stone Road West, Guelph, Ontario, Canada N1G 3W4. alfonso_valdivieso@phac-aspc.gc.ca

Journal of Food Protection

|March 8, 2007

Summary

A new hydrophobic grid membrane filter (HGMF) on semisolid medium (SSM) method simplifies thermophilic Campylobacter isolation from food. This antibiotic-free approach is faster, cheaper, and more efficient than traditional methods.

More Related Videos

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

Related Experiment Videos

Last Updated: Jul 16, 2026

Detection and Isolation of Campylobacter spp. from Raw Meat

Detection and Isolation of Campylobacter spp. from Raw Meat

Published on: February 23, 2024

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Culture Methods to Determine the Limit of Detection and Survival in Transport Media of Campylobacter Jejuni in Human Fecal Specimens

Published on: March 10, 2020

Area of Science:

Food microbiology
Food safety
Bacteriology

Background:

Traditional methods for isolating thermophilic Campylobacter from food are complex, labor-intensive, and time-consuming.
Existing methods often rely on selective antibiotics, which can inhibit the growth of competing microflora.
There is a need for simpler, faster, and more efficient isolation procedures.

Purpose of the Study:

To develop and evaluate a simplified procedure for isolating thermophilic campylobacters from food matrices.
To compare the efficacy of the new method with a standard antibiotic-containing procedure.

Main Methods:

A novel method using a hydrophobic grid membrane filter (HGMF) on antibiotic-free semisolid medium (SSM) was developed.
The HGMF physically separates bacteria, allowing isolation based on differential motility.
The HGMF-SSM method was compared to the Agriculture and Agri-Food Canada Food Safety Procedures Manual (FSPM-10) method using turkey, pig fecal, chicken, and turkey samples.

Main Results:

The HGMF-SSM method yielded pure Campylobacter cultures in 16–18 hours, compared to 48 hours for the FSPM-10 method.
The HGMF-SSM method showed higher recovery rates for thermophilic campylobacters (26.5% positive) compared to the FSPM-10 method (18.9% positive).
The HGMF-SSM method was found to be simpler, faster, cheaper, and less labor-intensive.

Conclusions:

The HGMF-SSM method offers an improved, more efficient approach for isolating thermophilic campylobacters from various food types.
The absence of antibiotics in the SSM may contribute to higher recovery rates.
The method's simplicity and efficiency have significant implications for food safety and epidemiological studies.