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Updated: Jul 16, 2026
Assay for Adhesion and Agar Invasion in S. cerevisiae
Published on: November 8, 2006
[Evaluation of Agar Candida ID2 (bioMerieux) a chromogenic medium for yeasts differentiation]
Emilia Ciok-Pater1, Patrycja Zalas, Eugenia Gospodarek
1Katedra i Zakład Mikrobiologii Collegium Medicum im. L. Rydygiera w Bydgoszczy, Uniwersytet Mikołaja Kopernika w Toruniu.
Abstract:
Invasive diagnostic and therapeutic methods, widespread antibiotic therapy and rising percent of the immunocompromised patients cause incrementation of frequency of occurrence of the yeast infection. C. albicans is the most commonly isolated species of Candida from clinical samples. However, recently growth of frequency of isolation Candida non - albicans from clinical specimens have been observed. Yeast-like fungi different from C. albicans have become serious clinical problem. Conventional methods of identification of the yeast-like fungi carry away a lot time enough. Employment of chromogenic agar shortens latency on result. We decided to examine the usefulness ofAgar Candida ID2 (CAN2) (bioMérieux) in the identification of Candida species. The subjects within the study were 146 of Candida spp strains which were isolated from the clinical specimens of patients hospitalized at the University Hospital in Bydgoszcz. Germ tube test. Api 20C AUX test (bioMérieux) and Agar Candida ID2 (bioMérieux) were used. We have ascertained correspondence of identifying species amounted to 82.2% of analyzed Candida species between API 20C AUX test and kind of growth on CAN2 medium. Divergence of results received between CAN2 medium and API 20C AUX test suggests necessity of conducting of verification data with other methods. In conclusion, our study shows that Agar Candida ID2 is an effective medium for the isolation yeast-like fungi and in preliminary identification of Candida species direct from clinical materials.
Insights
The Agar Candida ID2 (CAN2) medium effectively isolates yeast-like fungi and aids in preliminary identification of Candida species. This chromogenic agar offers a faster alternative to conventional methods for clinical samples.
Area of Science:
- Medical Mycology
- Clinical Microbiology
- Diagnostic Laboratory Science
Background:
- Increasing incidence of yeast infections due to invasive procedures, antibiotic use, and immunocompromised patients.
- Candida albicans is common, but non-albicans Candida species are emerging as significant clinical challenges.
- Conventional yeast identification methods are time-consuming.
Purpose of the Study:
- To evaluate the efficacy of Agar Candida ID2 (CAN2) for identifying Candida species.
- To compare CAN2 performance against standard methods like API 20C AUX and germ tube tests.
Main Methods:
- Utilized 146 Candida spp. strains from clinical specimens.
- Employed germ tube test, API 20C AUX, and Agar Candida ID2 (CAN2) for yeast identification.
- Assessed species identification correspondence between methods.
Main Results:
- Agar Candida ID2 (CAN2) demonstrated an 82.2% species identification correspondence with the API 20C AUX test.
- Discrepancies between CAN2 and API 20C AUX highlight the need for confirmatory testing.
- CAN2 proved effective for isolating yeast-like fungi directly from clinical materials.
Conclusions:
- Agar Candida ID2 (CAN2) is a valuable tool for the isolation and preliminary identification of Candida species.
- Chromogenic agar offers a reduced latency period compared to traditional identification techniques.
- Further verification with other methods is recommended when results from CAN2 and API 20C AUX diverge.
