Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium08:45

Establishment and Characterization of Patient-derived Gastric Organoids from Biopsies of Benign Gastric Body and Antral Epithelium

2.1K
Gastric patient-derived organoids find increasing use in research, yet formal protocols for generating human gastric organoids from single-cell digests with standardized seeding density are lacking. This protocol presents a detailed method for reliably creating gastric organoids from biopsy tissue obtained during upper...
2.1K
Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors05:16

Laparoscopy-endoscopy Cooperative Surgery for the Treatment of Gastric Gastrointestinal Stromal Tumors

6.6K
Laparoscopy-endoscopy cooperative surgery is appropriate for the treatment of gastric gastrointestinal stromal tumors less than 5 cm in size. It can achieve the respective advantages of endoscopic and laparoscopic surgeries while avoiding their...
6.6K
Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization08:16

Detection of G Protein-coupled Receptor Expression in Mouse Vagal Afferent Neurons using Multiplex In Situ Hybridization

4.6K
Multiplex in situ hybridization (ISH) was employed to simultaneously visualize the transcripts for two G protein-coupled receptors and one transcription factor in the entire vagal ganglionic complex of the adult mouse. This protocol could be used to generate accurate maps of the transcriptional profiles of vagal afferent...
4.6K
One-anastomosis Gastric Bypass (OAGB) in Rats08:16

One-anastomosis Gastric Bypass (OAGB) in Rats

11.5K
This protocol is for the performance of one-anastomosis gastric bypass (OAGB) on rat. The operator performs a long and tubular-stapled gastric pouch followed by hand-sewn anastomosis. For this model, the operator reproduces the same ratio between biliopancreatic and common limb in humans; therefore, the biliopancreatic limb measures 35 cm.
11.5K
Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test12:24

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

14.1K
Determination of gastric emptying with a non-invasive [13C]-octanoic acid breath test for tracking gastroparesis in female NOD LtJ...
14.1K
Roux-en-Y Gastric Bypass Operation in Rats07:37

Roux-en-Y Gastric Bypass Operation in Rats

24.3K
Numerous studies using gastric bypass rat models have been recently conducted to uncover the underlying physiological mechanisms of Roux-en-Y gastric bypass operations. This article aims to demonstrate and discuss the technical and experimental details of our published gastric bypass rat model to understand advantages and limitations of this experimental...
24.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determinants of Adherence to a Ketogenic Diet in Patients with Heart Failure with Reduced Ejection Fraction.

Nutrients·2026
Same author

PK-SMART: Penetrating Keratoplasty With Sutureless Membrane Artificial Endothelial Replacement Technique. Report of the First Three Clinical Cases.

Cornea·2026
Same author

Distinct Ocular Surface Microbial Profiles in Corneal Transplant Candidates.

Cornea·2026
Same author

Ciliochoroidal Effusion and Regional Scleral Thickening in Peripapillary Pachychoroid Syndrome.

American journal of ophthalmology·2026
Same author

Random Copolyester-Based Delivery Systems for Tear Protein Therapeutics in Ocular Surface Disorders.

ACS omega·2026
Same author

Distinct retinal remodeling and visual prognostic factors in advanced epiretinal membranes with and without associated retinal break.

BMC ophthalmology·2026
Same journal

Alcohol-Related Liver Disease: A Review.

JAMA·2026
Same journal

Online Prescribing of GLP-1 Receptor Agonists.

JAMA·2026
Same journal

Using AI Disagreement to Expose Gaps in Coverage Rules.

JAMA·2026
Same journal

Randomized Trials in Pregnancy.

JAMA·2026
Same journal

Randomized Trials in Pregnancy-Reply.

JAMA·2026
Same journal

WHO Issues Guidelines for Treating Ebola and Marburg Viruses.

JAMA·2026
See all related articles

Related Experiment Video

Updated: Jan 31, 2026

One-anastomosis Gastric Bypass OAGB in Rats
08:16

One-anastomosis Gastric Bypass OAGB in Rats

Published on: November 10, 2018

11.5K

Aging, adiposity, and calorie restriction.

Luigi Fontana1, Samuel Klein

  • 1Division of Geriatrics and Nutritional Science and Center for Human Nutrition, Washington University School of Medicine, St Louis, Mo 63110, USA. lfontana@im.wustl.edu

JAMA
|March 8, 2007
PubMed
Summary
This summary is machine-generated.

Calorie restriction in adults shows metabolic benefits similar to rodents, but optimal intake for human longevity remains unknown. Excessive restriction can cause malnutrition and adverse effects.

More Related Videos

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
12:24

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

Published on: March 23, 2013

14.1K
Roux-en-Y Gastric Bypass Operation in Rats
07:37

Roux-en-Y Gastric Bypass Operation in Rats

Published on: June 11, 2012

24.3K

Related Experiment Videos

Last Updated: Jan 31, 2026

One-anastomosis Gastric Bypass OAGB in Rats
08:16

One-anastomosis Gastric Bypass OAGB in Rats

Published on: November 10, 2018

11.5K
Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test
12:24

Assessment of Gastric Emptying in Non-obese Diabetic Mice Using a [13C]-octanoic Acid Breath Test

Published on: March 23, 2013

14.1K
Roux-en-Y Gastric Bypass Operation in Rats
07:37

Roux-en-Y Gastric Bypass Operation in Rats

Published on: June 11, 2012

24.3K

Area of Science:

  • Gerontology
  • Nutrition Science
  • Metabolic Health

Background:

  • Obesity from excessive calorie intake elevates chronic disease risk and reduces life expectancy.
  • Calorie restriction (CR) with adequate nutrients in rodents extends lifespan and lowers disease risk.

Purpose of the Study:

  • To evaluate the physiological and clinical implications of calorie restriction with adequate nutrient intake.

Main Methods:

  • Comprehensive literature search of PubMed, gerontology journals, and reference lists.
  • Inclusion of epidemiologic, case-control, and randomized controlled trials.
  • Quality assessment of reviewed reports based on peer-review, sample size, objectivity, and bias minimization.

Main Results:

  • CR in adult humans induces metabolic, hormonal, and inflammatory adaptations similar to CR rodents and monkeys.
  • These adaptations include reduced risk factors for diabetes, cardiovascular disease, and potentially cancer.
  • Excessive calorie restriction leads to malnutrition and adverse clinical outcomes.

Conclusions:

  • Calorie restriction in adults promotes beneficial metabolic, hormonal, and functional changes.
  • Optimal calorie intake and body fat for human health and longevity are not yet determined.
  • Moderate CR may pose risks for specific populations, such as lean individuals.