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[Identification of fetal nucleated erythrocytes in maternal blood using short tandem repeat typing after primer
Hong-ying Wei1, Gui-fang Long, Wei-xiong Lin
1Department of Pediatrics, The First Affiliated Hospital, Guangxi Medical University, Nanning 530021, China.
Objective:
To develop a method for identifying fetal nucleated erythrocytes (NRBCs) in maternal blood.
Methods:
NRBCs in maternal blood were detected by benzidine staining and collected by micromanipulation. After primer extension preamplification (PEP) of the entire genome from a single NRBC, short tandem repeat (STR) genotype was analysed after further amplification of this gene. Single NRBC was differentiated as fetal or maternal origin by comparison of STR genotype of NRBC with its corresponding parents.
Results:
NRBCs were found in all of 28 pregnant women in a range of 4 to 13 per 5 ml venous blood. About 43. 6% of NRBCs were determined to be fetal origin by STR typing.
Conclusion:
This method provides effective identification of fetal NRBCs and allows non-invasive prenatal genetic diagnosis using single fetal NRBC.
