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Efficient downregulation of alb1 gene using an AMA1-based episomal expression of RNAi construct in Aspergillus

Vahid Khalaj1, Hamid Eslami, Mohammad Azizi

  • 1Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. khalajs@pasteur.ac.ir

FEMS Microbiology Letters
|March 9, 2007
PubMed

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RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...