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Baoli Wang1, Fang Zheng, Jinxing Quan

  • 1Key Lab of Hormone and Development, Institute of Endocrinology, Tianjin Medical University, Tianjin 300070, People's Republic of China. zhe0412@yahoo.com.cn <zhe0412@yahoo.com.cn>

Analytical Biochemistry
|March 9, 2007
PubMed
Abstract

No abstract available in PubMed .

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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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