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Updated: Jan 10, 2026
Isolation of Murine Embryonic Hemogenic Endothelial Cells
Published on: June 17, 2016
Two conditional media promoting the differentiation murine embryonic stem cells into hematopoietic stem cells
Hui-Ping Zhao1, Guang-Xiu Lu, Qi-Ru Wang
1Institute of Human Reproductive and Stem Cell Engineering, Central South University, National Human Stem Cell Engineering Research Center, Changsha 410078, China.
Objective:
To derive hematopoietic stem cells with functional properties of hematopoietic reconstitution from murine embryonic stem (ES) cells.
Methods:
ES-D3 cells by formation of the day-4 embryoid bodies (4dEBs) were induced into hematopoietic stem cells by co-culture with murine bone marrow endothelial cell-conditional medium (mBMEC-CM) and the fetal liver stromal cell-conditional medium (FLSC-CM). This experiment was designed to 4 groups (mBMEC-CM + FLSC-CM group, mBMEC-CM group, FLSC-CM group, and the control group).
Results:
The total cell numbers, CD34+ cell numbers, and colony numbers formed in the mBMEC-CM+FLSC-CM group were the highest among the 4 groups. The cells in the mBMEC-CM + FLSC-CM group resumed the hematopoietic system of the mice after being transplanted with the inducing cells.
Conclusion:
The culture condition combing mBMEC-CM with FLSC-CM can promote murine ES cells differentiating into hematopoietic stem cells with functional properties of hematopoietic reconstitution.
