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Updated: Jul 16, 2026
Murine Model of Intestinal Ischemia-reperfusion Injury
Published on: May 11, 2016
Mesenteric ischemia/reperfusion-induced intestinal and vascular damage: effect of stobadine
V Nosál'ová1, J Navarová, D Mihalová
1Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic. exfanosv@savba.sk
Abstract:
This study examined the effects of the pyridoindole compound stobadine on intestinal and vascular injury following mesenteric ischemia/reperfusion (I/R) in rats. Ischemia was induced by occlusion of the superior mesenteric artery (SMA) for 60 min, followed by 30 min reperfusion. To characterize gut impairment, some parameters of intestinal damage and biochemical variables, such as GSH content, activity of a lysosomal enzyme N-acetyl-beta-D-glucuronidase and activity of gamma-glutamyl transpeptidase, were determined. Vascular I/R-induced damage was evaluated as changes in acetylcholine evoked relaxation of mesenteric artery rings under isometric conditions. A method of amplified chemiluminescence (CL) was used to detect production of reactive oxygen species (ROS). Following I/R, pronounced intestinal injury of various intensities was observed, with maximal changes occurring in the terminal ileum. The effect of I/R was expressed mainly as increased vascular permeability, with protein leakage and subsequent hemorrhagic injury of the intestine as well as impaired endothelium-dependent SMA relaxation. Vessel dysfunction was manifested by a decrease of the maximal relaxation response to acetylcholine. An increase of CL, indicative of increased ROS production, was observed in both intestinal and vascular tissue. A novel antioxidant, stobadine, was found to reduce the increased vascular permeability and the extent of small intestine injury caused by I/R, to improve biochemical alterations accompanying I/R, to protect endothelial-dependent relaxation of mesenteric arteries, and to attenuate the CL response. The observed beneficial effect of stobadine indicates its possible application in the preventive and/or therapeutic approach to I/R-induced pathologies.
Insights
Stobadine, a novel antioxidant, effectively reduced intestinal and vascular injury caused by mesenteric ischemia/reperfusion (I/R) in rats. This compound protected against reactive oxygen species (ROS) damage and improved biochemical markers, showing therapeutic potential.
Area of Science:
- Pharmacology
- Gastroenterology
- Cardiovascular Science
Background:
- Mesenteric ischemia/reperfusion (I/R) induces significant intestinal and vascular damage.
- Reactive oxygen species (ROS) play a critical role in I/R-induced pathologies.
- Current therapeutic strategies for I/R injury have limitations.
Purpose of the Study:
- To investigate the protective effects of stobadine against mesenteric I/R injury in a rat model.
- To evaluate stobadine's impact on intestinal barrier function, biochemical markers, and vascular reactivity.
- To assess stobadine's influence on ROS production in I/R-affected tissues.
Main Methods:
- Induction of mesenteric ischemia (60 min) and reperfusion (30 min) in rats.
- Assessment of intestinal injury parameters including permeability and biochemical markers (GSH, N-acetyl-beta-D-glucuronidase, gamma-glutamyl transpeptidase).
- Evaluation of vascular function via acetylcholine-induced relaxation of mesenteric artery rings and measurement of ROS using chemiluminescence.
Main Results:
- I/R led to pronounced intestinal injury, increased vascular permeability, hemorrhagic damage, and impaired endothelium-dependent vasodilation.
- ROS production, indicated by chemiluminescence, increased in both intestinal and vascular tissues post-I/R.
- Stobadine administration significantly reduced I/R-induced intestinal injury, improved biochemical parameters, preserved vascular function, and attenuated ROS production.
Conclusions:
- Stobadine demonstrates significant protective effects against mesenteric I/R-induced intestinal and vascular injury in rats.
- The antioxidant properties of stobadine contribute to its beneficial effects by mitigating ROS-mediated damage.
- Stobadine holds promise as a potential therapeutic agent for preventing or treating I/R-related conditions.
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