Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A high density barley microsatellite consensus map with 775 SSR loci.

R K Varshney1, T C Marcel, L Ramsay

  • 1Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany. r.k.varshney@cgiar.org

TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik
|March 9, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Developing robust food composition models: Strategies for handling temperature and packaging variations in dry-cured ham using near infrared spectrometry.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

What is the medical requirement for a quick release system in a body armour vest?

BMJ military health·2024
Same author

Building a Self-Sustaining Psychology Research Team in Academic Medicine: A Multi-Tiered Mentorship Model.

Journal of clinical psychology in medical settings·2024
Same author

Seafloor macrolitter as a settling platform for non-native species: A case study from UK waters.

Marine pollution bulletin·2024
Same author

Vocal and tongue exercise in early to mid-stage Parkinson disease using the Pink1-/- rat.

Brain research·2024
Same author

The 'Wirral Wedge': an aid to position arm safely in upper limb surgery.

Annals of the Royal College of Surgeons of England·2024

A high-density consensus map of barley microsatellite markers was created by integrating six genetic maps. This tool aids barley molecular breeding with improved marker selection and higher polymorphism probability.

Area of Science:

  • Plant Genetics
  • Molecular Biology
  • Genomics

Background:

  • Microsatellite or simple sequence repeat (SSR) markers are crucial for genetic mapping.
  • Previous genetic maps of barley lacked comprehensive integration and high marker density.

Purpose of the Study:

  • To construct a high-density consensus SSR map of barley by integrating multiple independent genetic maps.
  • To provide a valuable resource for barley molecular breeding programs and future genomic studies.

Main Methods:

  • Integrated six barley genetic maps using segregation data from various research groups.
  • Employed computer programs RECORD and JoinMap 3.0 for marker ordering and map construction.
  • Utilized a "neighbours" map approach for final map calculation.

Related Experiment Videos

Main Results:

  • Developed an integrated barley SSR consensus map with 775 unique loci from 688 primer pairs.
  • Achieved an average marker density of one SSR marker every 1.38 cM across 1,068 cM.
  • Genomic DNA-derived SSR loci exhibited higher polymorphism information content (0.61) than EST/gene-derived loci (0.48).

Conclusions:

  • The high-density consensus SSR map enhances marker selection and polymorphism probability for barley breeding.
  • This map facilitates alignment with physical maps and aids in haplotype diversity studies.