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Published on: May 1, 2014
A high density barley microsatellite consensus map with 775 SSR loci.
R K Varshney1, T C Marcel, L Ramsay
1Leibniz-Institute of Plant Genetics and Crop Plant Research (IPK), Corrensstrasse 3, 06466 Gatersleben, Germany. r.k.varshney@cgiar.org
Summary
A high-density consensus map of barley microsatellite markers was created by integrating six genetic maps. This tool aids barley molecular breeding with improved marker selection and higher polymorphism probability.
Area of Science:
- Plant Genetics
- Molecular Biology
- Genomics
Background:
- Microsatellite or simple sequence repeat (SSR) markers are crucial for genetic mapping.
- Previous genetic maps of barley lacked comprehensive integration and high marker density.
Purpose of the Study:
- To construct a high-density consensus SSR map of barley by integrating multiple independent genetic maps.
- To provide a valuable resource for barley molecular breeding programs and future genomic studies.
Main Methods:
- Integrated six barley genetic maps using segregation data from various research groups.
- Employed computer programs RECORD and JoinMap 3.0 for marker ordering and map construction.
- Utilized a "neighbours" map approach for final map calculation.
Main Results:
- Developed an integrated barley SSR consensus map with 775 unique loci from 688 primer pairs.
- Achieved an average marker density of one SSR marker every 1.38 cM across 1,068 cM.
- Genomic DNA-derived SSR loci exhibited higher polymorphism information content (0.61) than EST/gene-derived loci (0.48).
Conclusions:
- The high-density consensus SSR map enhances marker selection and polymorphism probability for barley breeding.
- This map facilitates alignment with physical maps and aids in haplotype diversity studies.
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