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Related Experiment Videos

[Transglycosylation of L-ascorbic acid].

A A Markosian, L A Abelian, M O Adamian

    Prikladnaia Biokhimiia I Mikrobiologiia
    |March 10, 2007
    PubMed
    Summary
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    Cyclodextrin glucanotransferases (CGTase) and maltase enzymes were used for transglycosylation of L-ascorbic acid. Thermophilic CGTases showed the highest efficiency, achieving over 60% transglucosylation.

    Area of Science:

    • Biochemistry
    • Enzymology
    • Carbohydrate Chemistry

    Background:

    • L-ascorbic acid (Vitamin C) is a vital nutrient with limited stability.
    • Enzymatic modification offers a route to enhance the stability of L-ascorbic acid.
    • Cyclodextrin glucanotransferases (CGTase) and maltase are enzymes capable of transferring glycosyl residues.

    Purpose of the Study:

    • To investigate the transglycosylation of L-ascorbic acid using various glycosyl donors.
    • To compare the efficiency of CGTases from different bacterial sources (mesophilic, thermophilic, halophilic) and maltase from Saccharomyces cerevisiae.
    • To determine the optimal conditions for enhancing L-ascorbic acid stability through enzymatic modification.

    Main Methods:

    • Enzymatic transglycosylation reactions were performed using L-ascorbic acid as the acceptor.

    Related Experiment Videos

  • Glycosyl donors included starch, maltodextrin, gamma-cyclodextrin, and maltose.
  • Cyclodextrin glucanotransferases (CGTase) from mesophilic, thermophilic, and halophilic bacilli, and maltase from Saccharomyces cerevisiae were utilized.
  • Reaction products were analyzed to determine the degree of transglucosylation.

    • Main Results:

    • Cyclodextrin glucanotransferases (CGTase) and maltase effectively catalyzed the transglycosylation of L-ascorbic acid.
    • CGTases derived from thermophilic bacilli demonstrated superior efficiency compared to mesophilic and halophilic counterparts.
    • Transglucosylation degrees exceeding 60% were achieved, indicating significant modification of L-ascorbic acid.

    Conclusions:

    • Thermophilic bacterial cyclodextrin glucanotransferases are highly effective biocatalysts for the synthesis of L-ascorbic acid derivatives.
    • Enzymatic transglycosylation presents a promising strategy for improving the stability and potentially the bioavailability of L-ascorbic acid.
    • Further research into optimizing these enzymatic processes could lead to enhanced Vitamin C formulations.