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REMA: A computer-based mapping tool for analysis of restriction sites in multiple DNA sequences
Jan Szubert1, Caroline Reiff, Andrew Thorburn
1Faculty of Life Sciences, The University of Manchester, Oxford Road, Manchester, M13 9PT UK.
Journal of Microbiological Methods
|March 10, 2007
Summary
REMA is a web-based tool that predicts DNA endonuclease cut sites, analyzing multiple sequences to determine fragment sizes and generate restriction maps. This program aids molecular biology research by simplifying DNA analysis.
Area of Science:
- Bioinformatics
- Molecular Biology
Background:
- REMA is a freely available, web-based program for analyzing DNA sequences.
- It is written in Perl and accessible for non-commercial use.
Purpose of the Study:
- To predict endonuclease cut sites in DNA sequences.
- To provide restriction maps and analyze DNA fragments.
- To assist in selecting restriction enzymes for discriminatory analysis.
Main Methods:
- Interactive web-based analysis of multiple DNA sequences.
- Prediction of endonuclease cut sites.
- Calculation of fragment number and size.
- Generation of restriction maps.
Main Results:
- REMA predicts endonuclease cut sites in DNA.
- It analyzes multiple sequences simultaneously.
- The program provides fragment number, size, and restriction maps.
- It suggests enzymes for discriminatory analysis.
Conclusions:
- REMA is an easy-to-use tool for DNA sequence analysis.
- It has broad applications in molecular biology research.
- The program aids in predicting restriction enzyme activity and fragment generation.
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