Gastrin release from antral G cells stimulated with secretin

Recently, gastrinoma cells were demonstrated to release gastrin when directly stimulated by secretin both in vivo and in vitro. In this study, the reaction of antral G cells was investigated. Secretin was injected into the right gastroepiploic artery in canines, and into the common hepatic artery during a selective arteriography in patients without gastrinomas. G cells obtained from the antrum of rats were attached to 0.45-microns filters and irrigated with medium containing secretin. The serum gastrin concentration increased rapidly in significant amounts and very quickly after an intraarterial injection of secretin, both in humans and in dogs. The rate of gastrin release from the rat antral G cells in vitro increased significantly when the medium contained secretin. In conclusion, secretin stimulated gastrin release from antral G cells both in vivo and in vitro.