Related Experiment Video

Updated: Jul 16, 2026

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung
15:01

Confocal Laser Scanning Microscopy-Based Quantitative Analysis of Aspergillus fumigatus Conidia Distribution in Whole-Mount Optically Cleared Mouse Lung

Published on: September 18, 2021

[Allergen activity of xerophilic fungus, Aspergillus restrictus]

Takeshi Itabashi1, Tomoo Hosoe, Norihiro Toyasaki

  • 1Hoshi University Faculty of Pharmaceutical sciences. itabashi@hoshi.ac.jp

Arerugi = [Allergy]
|March 10, 2007
PubMed
Abstract

Insights

Aspergillus restrictus, a xerophilic fungus found in house dust, may trigger adult asthma. Allergen preparation methods and cross-reactivity with Aspergillus fumigatus influence its impact.

Area of Science:

  • Mycology
  • Immunology
  • Environmental Health

Context:

  • Xerophilic fungi, like Aspergillus restrictus, are common in house dust.
  • Fungal allergen composition is influenced by culture and extraction conditions.

Purpose:

  • To investigate Aspergillus restrictus as a potential allergen in adult asthma.
  • To explore the impact of different extraction methods on A. restrictus allergenicity.
  • To analyze cross-reactivity between A. restrictus and A. fumigatus.

Summary:

  • Four extracts of A. restrictus were tested for specific IgE in asthma patients' sera.
  • ELISA inhibition revealed cross-reactivity between A. restrictus and A. fumigatus.
  • Component analysis identified allergens in A. restrictus via SDS-PAGE and Western Blotting.

Impact:

  • A. restrictus is indicated as a potential causative agent in adult asthma.
  • Extraction method significantly influences allergen activity.
  • Cross-reactivity with A. fumigatus suggests shared allergenic components.

More Related Videos

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis09:52

Histological Quantification to Determine Lung Fungal Burden in Experimental Aspergillosis

Published on: March 9, 2018

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus12:29

Live Imaging of Antifungal Activity by Human Primary Neutrophils and Monocytes in Response to A. fumigatus

Published on: April 19, 2017

Related Concept Videos

Fungal Phylum Ascomycota01:28

Fungal Phylum Ascomycota

Phylum Ascomycota, a major division within the subkingdom Dikarya, comprises a diverse range of fungal species, including both unicellular yeasts and filamentous molds such as Aspergillus and Penicillium. These fungi thrive in a variety of habitats, from aquatic ecosystems to terrestrial environments, playing crucial ecological and economic roles.Morphology and ReproductionThe defining characteristic of Ascomycetes, commonly referred to as sac fungi, is the ascus—a sac-like structure that...
Fungal Group Zygomycota01:29

Fungal Group Zygomycota

Zygomycota, previously classified as a distinct fungal group, are primarily terrestrial, saprophytic molds that play a crucial role as decomposers. Recent phylogenetic studies have revealed that these fungi are now divided into two major clades — Mucoromycota, which includes many symbiotic species, and Zoopagomycota, which primarily consists of parasitic and pathogenic fungi. These groups exhibit distinct ecological roles and reproductive strategies while sharing key structural and...
Antifungal Agents01:15

Antifungal Agents

Amphotericin B is a broad-spectrum antifungal agent that exploits structural differences between fungal and mammalian cell membranes. Its amphipathic structure—featuring a hydrophobic polyene-lactone ring and a hydrophilic region containing mycosamine and carboxylic acid groups—enables selective binding to ergosterol, a sterol predominantly found in fungal plasma membranes. This selective interaction underlies the drug’s antifungal activity, although weak binding to cholesterol contributes to...
Allergic Reactions02:06

Allergic Reactions

Overview
Cross-reactivity00:42

Cross-reactivity

Overview