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[Studies on diterpenes in needles of Pinus sylvestris]
Rong Zhang1, Hong-quan Duan, Zhi Yao
1The School of Pharmacy, Tianjin Medical University, Tianjin 300070, China.
Objective:
To research the constituents in needles of Pinus sylvestris.
Method:
Repeated column chromatography and preparation HPLC are used for compound isolation, and their structures were elucidated on the basis of spectral data analysis.
Result:
Six compounds, pinifolic acid (1), 15-oxo-8 (17) -labden-18-oic acid (2) , 15-acetoxy-labd-8 ( 17)-en-18-oic acid (3), dehydroabietic acid (4), 7alpha-hydroxydehydroabietic acid (5), 7beta-hydroxydehydroabietic acid (6) were isolated from the needles of P. sylvestris.
Conclusion:
Compound 3-6 were isolated from the needles of P. sylvestris for the first time, and compound 3 is a new natural product. The petroleum ether and EtOAc extracts showed significant cytotoxic effects to Hela and A549. Compounds 2, 4-6 revealed a positive distinction compared to the control group.
