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Updated: Jul 16, 2026
Leaf Spray Mass Spectrometry: A Rapid Ambient Ionization Technique to Directly Assess Metabolites from Plant Tissues
Published on: June 21, 2018
[Studies on chemical constituents from leaves of Isatis indigotica]
Ji-feng Liu1, Xue-mei Zhang, Duo-qing Xue
1State Key Labortatory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, the Chinese Academy of Sciences, Kunming 650204, China.
Objective:
To study the chemical constituents of the leaves of Isatis indigotica.
Method:
The leaves of I. indigotica were extracted with 80% ethanol. The EtOH extract was dispersed in H20 and extracted with petroleum, EtOAc and BuOH successively. The EtOAc fraction was isolated and purified by column chromatography on silica gel, Sephadex LH -20 and Rp-8, Rp-18. All the compounds were identified on the basis of spectral analyses (including MS, 1H-NMR, 13 C-NMR) , RESULT: Eleven compounds were isolated from the leaves of I. indigotica, and structures were characterized as 10H-indolo [3, 2-b] quinoline (1), indirubin (2), 4 (3H)-quinazo-linone (3), (E)-3-(3', 5'-dimethoxy-4'-hydroxybenzylidene) -2-indolinone (4), 2, 3-dihydropyrrolo [2, 1-b] quinazolin-9(1H) -one (5) , benzoic acid (6) , o-droxy-benzoic acid (7), ( - ) -lariciresinol (8) , ( + ) -isolariciresinol (9), isovitexin (10), 6-f-D-glucopyranosyldiosmetin (11).
Conclusion:
1, 4, 5, 8, 9, 11 were obtained from the leaves of I. indigotica for the first time.
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