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Characterization of Human Monocyte Subsets by Whole Blood Flow Cytometry Analysis
Published on: October 17, 2018
[Study on monocyte HLA-DR expression in critically ill patients after surgery]
1The Department of ICU, the First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou, China.
Zhonghua Wai Ke Za Zhi [Chinese Journal of Surgery]
|March 14, 2007
Summary
In critically ill patients, low HLA-DR expression on monocytes post-surgery is not a reliable prognosis indicator. However, increasing monocyte HLA-DR expression by day 7 suggests a better 28-day survival outcome.
Area of Science:
- Immunology
- Critical Care Medicine
- Clinical Pathology
Background:
- Critically ill patients post-surgery often exhibit immune dysregulation.
- Monocytes play a crucial role in the immune response and their function can be assessed by HLA-DR expression.
Purpose of the Study:
- To investigate Human Leukocyte Antigen - Antigen D Related (HLA-DR) expression on CD14(+) monocytes in critically ill surgical patients.
- To determine the relationship between monocyte HLA-DR expression and patient prognosis.
Main Methods:
- Flow cytometry was used to measure peripheral blood CD14(+) monocyte HLA-DR expression on days 1 and 7 post-ICU admission in 30 surgical patients and 28 healthy controls.
- APACHE II, SOFA scores, age, sex, and 28-day survival were recorded.
Main Results:
- Patients had significantly lower monocyte HLA-DR expression compared to healthy volunteers (P < 0.01).
- Day 1 HLA-DR expression did not correlate with APACHE II, SOFA scores, or 28-day prognosis.
- Increased monocyte HLA-DR expression by day 7 was associated with improved 28-day survival (P < 0.01).
Conclusions:
- Decreased monocyte HLA-DR on day 1 post-ICU is not a prognostic marker in critically ill surgical patients.
- Monitoring the increase in monocyte HLA-DR expression by day 7 is significant for evaluating 28-day prognosis.
