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Radiation-enhanced murine sarcoma virus genome rescue activity
Zhonghua Minguo Wei Sheng Wu Xue Za Zhi = Chinese Journal of Microbiology
|June 1, 1975
Summary
X-irradiation of Moloney murine leukemia virus (MuLV)- carrier cells significantly enhances Moloney sarcoma virus (MSV) retrieval from nonproducer cells. This suggests MuLV-carrier cell viability is crucial for MSV genome rescue.
Area of Science:
- Virology
- Molecular Biology
- Cancer Research
Background:
- X-irradiation of cells impairs DNA synthesis and replication.
- Murine leukemia virus (MuLV) carrier cells and Moloney sarcoma virus (MSV)-induced tumor nonproducer cells are relevant models for studying viral genome rescue.
Purpose of the Study:
- To investigate the effect of X-irradiation on MSV genome rescue efficiency.
- To determine the role of MuLV-carrier cells and nonproducer cells in MSV retrieval.
- To elucidate the mechanism of MSV genome rescue enhancement.
Main Methods:
- Co-cultivation of X-irradiated MuLV-carrier cells with un-irradiated nonproducer cells.
- Uridine-3H-labeling and focus assay experiments.
- Analysis of MSV/MuLV ratio in culture fluids.
Main Results:
- Co-cultivation of X-irradiated MuLV-carrier cells with un-irradiated nonproducer cells resulted in a 20-fold increase in MSV retrieval.
- A 3-fold increase in MSV-producing cells (infectious centers) was observed.
- Pre-irradiation of MuLV-carrier cells increased the MSV/MuLV ratio, while X-irradiation of nonproducer cells reduced MSV genome rescue efficiency.
Conclusions:
- X-irradiation of MuLV-carrier cells enhances MSV genome rescue efficiency.
- Viability of nonproducer cells is essential for MuLV replication and subsequent MSV genome rescue.
- Co-cultivation strategies involving irradiated MuLV-carrier cells can improve MSV retrieval.