Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

c-Src activates endonuclease-mediated mRNA decay.

Yong Peng1, Daniel R Schoenberg

  • 1Department of Molecular and Cellular Biochemistry, The RNA Group and the Comprehensive Cancer Center, The Ohio State University, Columbus, OH 43210, USA.

Molecular Cell
|March 14, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Analyzing (Re)Capping of mRNA Using Transcript Specific 5' End Sequencing.

Bio-protocol·2021
Same author

Cytoplasmic mRNA recapping has limited impact on proteome complexity.

Open biology·2020
Same author

Inhibition of cytoplasmic cap methylation identifies 5' TOP mRNAs as recapping targets and reveals recapping sites downstream of native 5' ends.

Nucleic acids research·2020
Same author

Assessment of hearing loss induced by tympanic membrane perforations under blast environment.

European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery·2019
Same author

Jumonji domain-containing 6 (JMJD6) identified as a potential therapeutic target in ovarian cancer.

Signal transduction and targeted therapy·2019
Same author

Atomic Arrangement in Metal-Doped NiS<sub>2</sub> Boosts the Hydrogen Evolution Reaction in Alkaline Media.

Angewandte Chemie (International ed. in English)·2019
Same journal

Thyroid cancer-associated EZH1 Q571R mutation drives chromatin compaction and H3K27me3 invasion into active chromatin.

Molecular cell·2026
Same journal

Genome-wide rotational and translational phasing of nucleosomes with human transcription factors.

Molecular cell·2026
Same journal

Spliceosomal proofreading factors safeguard 3' splice-site fidelity and prevent proteotoxicity and inflammation.

Molecular cell·2026
Same journal

Cytosolic EZH2-IMPDH2 complexes regulate melanoma progression and metastasis via GTP.

Molecular cell·2026
Same journal

A bacterial reverse transcriptase: Protein-templated DNA synthesis fuels antiviral immunity.

Molecular cell·2026
Same journal

Tweezing apart ribosome heterogeneity.

Molecular cell·2026
See all related articles

The protein c-Src activates mRNA decay by phosphorylating PMR1, a key enzyme in the process. This discovery links signal transduction pathways to mRNA degradation.

Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • The mRNA endonuclease PMR1 initiates mRNA decay.
  • PMR1's targeting to polysomes and decay activation depend on tyrosine phosphorylation at position 650.

Purpose of the Study:

  • To identify the kinase responsible for PMR1 phosphorylation.
  • To elucidate the mechanism linking signal transduction to mRNA decay.

Main Methods:

  • Immunoprecipitation to detect PMR1-c-Src complex formation.
  • In vitro and in vivo phosphorylation assays.
  • Analysis of PMR1 activity in cells with varying c-Src levels.
  • Epidermal Growth Factor (EGF) stimulation experiments.

Main Results:

Related Experiment Videos

  • c-Src was identified as the kinase phosphorylating PMR1.
  • c-Src interacts with PMR1 via Y650 and N-terminal SH3-binding motifs.
  • Active c-Src is required for PMR1 polysome targeting and mRNA decay.
  • EGF stimulation rapidly induces c-Src-mediated PMR1 phosphorylation.

Conclusions:

  • c-Src is the specific kinase that activates the PMR1-mediated mRNA decay pathway.
  • This study establishes a direct link between tyrosine kinase signaling and mRNA degradation processes.