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Updated: Jul 16, 2026
Standardized Rat Coronary Ring Preparation and Real-Time Recording of Dynamic Tension Changes Along Vessel Diameter
Published on: June 16, 2022
Ostreolysin affects rat aorta ring tension and endothelial cell viability in vitro
Katja Rebolj1, Urska Batista, Kristina Sepcić
1Department of Biology, Biotechnical Faculty, University of Ljubljana, Vecna pot 111, 1000 Ljubljana, Slovenia.
Abstract:
The effects of ostreolysin, a cardiotoxic cytolysin produced by the edible oyster mushroom (Pleurotus ostreatus), were studied on tension development in isolated rat aortic ring. Its cytotoxic effects on human umbilical vein endothelial cells and Chinese hamster lung fibroblasts were also studied. Ostreolysin induced a concentration-dependent increase in aortic ring tension in the range from 5 to 30 microg/ml, and was cytotoxic to both cell lines. These effects could contribute significantly to its previously observed cardiotoxicity.