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Related Concept Videos

Exon Recombination02:32

Exon Recombination

The evolution of new genes is critical for speciation. Exon recombination, also known as exon shuffling or domain shuffling, is an important means of new gene formation. It is observed across vertebrates, invertebrates, and in some plants such as potatoes and sunflowers. During exon recombination, exons from the same or different genes recombine and produce new exon-intron combinations, which might evolve into new genes. 
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What is Gene Expression?01:36

What is Gene Expression?

A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised  of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then processed and...
What is Gene Expression?01:42

What is Gene Expression?

Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
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Ribosomal RNA Synthesis02:53

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lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...
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lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA (lncRNA)...

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Related Experiment Video

Updated: Jul 16, 2026

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation
08:44

Identification and Characterization of Immunogenic RNA Species in HDM Allergens that Modulate Eosinophilic Lung Inflammation

Published on: May 30, 2020

EGO, a novel, noncoding RNA gene, regulates eosinophil granule protein transcript expression.

Lori A Wagner1, Clarissa J Christensen, Diane M Dunn

  • 1School of Medicine, Department of Dermatology, University of Utah, Salt Lake City, Utah 84132, USA. lori.wagner@hsc.utah.edu

Blood
|March 14, 2007
PubMed
Summary

A novel noncoding RNA, EGO (eosinophil granule ontogeny), is crucial for eosinophil development. Its expression is essential for producing key eosinophil proteins like MBP and EDN.

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Last Updated: Jul 16, 2026

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11:19

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Published on: September 30, 2016

Area of Science:

  • Hematology
  • Molecular Biology
  • RNA Biology

Background:

  • Eosinophil development involves complex gene regulation.
  • Proinflammatory cytokines and chemokines play roles in eosinophil differentiation.
  • The function of many noncoding RNAs (ncRNAs) in hematopoiesis remains largely uncharacterized.

Purpose of the Study:

  • To identify and characterize novel genes involved in early eosinophil development.
  • To investigate the role of a newly discovered gene, EGO, in eosinophil differentiation.
  • To determine the functional significance of EGO in the expression of eosinophil granule proteins.

Main Methods:

  • Gene expression profiling of developing eosinophils.
  • RNA sequencing and bioinformatics analysis to identify novel transcripts.
  • Functional studies using RNA silencing in hematopoietic progenitor cells.
  • Quantitative reverse transcription PCR (qRT-PCR) to measure gene expression levels.

Main Results:

  • A novel ncRNA, EGO (eosinophil granule ontogeny), was identified and found to be upregulated during early eosinophil development.
  • EGO is transcribed from an intronic region of the ITPRI gene and is not associated with ribosomes.
  • IL-5 stimulation significantly increased EGO transcript levels in CD34(+) hematopoietic progenitors.
  • RNA silencing of EGO led to reduced mRNA expression of major basic protein (MBP) and eosinophil-derived neurotoxin (EDN) in developing eosinophils.

Conclusions:

  • EGO is a novel ncRNA essential for eosinophil development.
  • EGO plays a critical role in regulating the expression of key eosinophil granule proteins, MBP and EDN.
  • EGO represents a new target for understanding and potentially modulating eosinophil-related inflammatory responses.