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Updated: Jul 16, 2026

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks
11:04

Inducible LAP-tagged Stable Cell Lines for Investigating Protein Function, Spatiotemporal Localization and Protein Interaction Networks

Published on: December 24, 2016

LAPSER1 is a putative cytokinetic tumor suppressor that shows the same centrosome and midbody subcellular

Haruka Sudo1, Yoshiro Maru

  • 1Department of Pharmacology, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo 162-8666, Japan.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|March 14, 2007
PubMed

Insights

LAPSER1, a potential tumor suppressor, is crucial for cell division in prostate cancer. Its disruption leads to abnormal cell structures and potential genetic instability, offering new therapeutic targets.

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Genetics

Background:

  • Prostate cancer is a leading cause of cancer death in men worldwide.
  • Current treatments for prostate cancer have limited efficacy, necessitating the search for novel therapeutic strategies.
  • The tumor suppressor role of LAPSER1 in prostate cancer is suspected but not fully understood.

Purpose of the Study:

  • To elucidate the function of LAPSER1 in prostate cancer cells.
  • To investigate the role of LAPSER1 in cell division and cytokinesis.
  • To explore the potential of LAPSER1 as a therapeutic target for prostate cancer.

Main Methods:

  • Immunofluorescence microscopy to determine LAPSER1 localization in mitotic cells.
  • RNA interference (RNAi) to disrupt LAPSER1 expression.
  • Analysis of cell morphology, binucleation, and mitotic catastrophe.
  • Investigation of LAPSER1 interaction with p80 katanin.

Main Results:

  • LAPSER1 localizes to centrosomes and midbodies, interacting with key proteins involved in cell division.
  • Disruption of LAPSER1 leads to defects in the central spindle and cytokinesis, resulting in binucleation.
  • Overexpression of LAPSER1 confers resistance to oncogenic transformation and induces mitotic catastrophe in a p80 katanin-dependent manner.
  • LAPSER1's interaction with p80 katanin is essential for proper cytokinesis.

Conclusions:

  • LAPSER1 plays a critical role in cytokinesis, likely through its interaction with p80 katanin.
  • Disruption of LAPSER1 function can lead to genetic instability, a hallmark of cancer.
  • LAPSER1 represents a promising novel target for prostate cancer therapy.

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