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Updated: Jul 16, 2026

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
Published on: November 2, 2009
Reporter proteins for in vivo fluorescence without oxygen
Thomas Drepper1, Thorsten Eggert, Franco Circolone
1Institute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Research Center Juelich, Stetternicher Forst, D-52426 Juelich, Germany.
Abstract:
Fluorescent reporter proteins such as green fluorescent protein are valuable noninvasive molecular tools for in vivo real-time imaging of living specimens. However, their use is generally restricted to aerobic systems, as the formation of their chromophores strictly requires oxygen. Starting with blue-light photoreceptors from Bacillus subtilis and Pseudomonas putida that contain light-oxygen-voltage-sensing domains, we engineered flavin mononucleotide-based fluorescent proteins that can be used as fluorescent reporters in both aerobic and anaerobic biological systems.
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