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Updated: Jul 16, 2026
AMEBaS: Automatic Midline Extraction and Background Subtraction of Ratiometric Fluorescence Time-Lapses of Polarized Single Cells
Published on: June 23, 2023
Accelerated multiple-pass moving average: a novel algorithm for baseline estimation in CE and its application to
Alejandro Solis1, Mathew Rex, Andres D Campiglia
1Postgrado de Instrumentación, Escuela de Física, Facultad de Ciencias, Universidad Central de Venezuela, Apartado, Los Chaguaramos, Caracas, Venezuela.
Abstract:
We present a novel algorithm for baseline estimation in CE. The new algorithm which we have named as accelerated multiple-pass moving average (AMPMA) is combined to three preexisting low-pass filters, spike-removal, moving average, and multi-pass moving average filter, to achieve real-time baseline correction with commercial instrumentation. The successful performance of AMPMA is demonstrated with simulated and experimental data. Straightforward comparison of experimental data clearly shows the improvement AMPMA provides to the linear fitting, LOD, and accuracy (absolute error) of CE analysis.
