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Updated: Jul 16, 2026
Mouse Models Of Helicobacter Infection And Gastric Pathologies
Published on: October 18, 2018
Adherence and invasion of mouse-adapted H pylori in different epithelial cell lines
Mao-Jun Zhang1, Fan-Liang Meng, Xiao-Yun Ji
1Department of Diagnostics, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, PO Box 5, Changping, Beijing 102206, China.
Aim:
To assess the adhesion and invasion abilities of different mouse adapted H pylori strains in different cell lines in vitro and investigate their effects on the virulence factors cagA and vacA.
Methods:
The adherence and invasion abilities of different H pylori strains in different epithelial cell lines were examined by the gentamycin protection assay. The null mutants of cagA and vacA were processed by direct PCR mutation method. The morphologic changes of different cell lines after H pylori attachment were examined by microscopy.
Results:
The densities of adherence to and invasion into cells in vitro were different from those in the mouse infection experiments. 88-3887 strain could invade and adhere to cells stronger than SS1 and X47. All tested strains had better adhering and invasive abilities in SCG-7901 cell. CagA and vacA minus mutants had the same invasion and adherent abilities as their wild types. In all strains and cell lines tested, only AGS cell had the significant hummingbird phenotype after inoculation with the 88-3887 wild-type.
Conclusion:
Both the host cells and the bacteria play important parts in the invasion and adhesion abilities of H pylori. CagA and VacA are not related to the ability of invasion and adhesion of H pylori in different cell lines in vitro.
Insights
Mouse-adapted Helicobacter pylori (H pylori) strains show varied adhesion and invasion in cell lines. Virulence factors CagA and VacA do not influence H pylori invasion or adhesion in vitro.
Area of Science:
- Microbiology
- Cell Biology
- Infectious Diseases
Background:
- Helicobacter pylori (H pylori) is a significant human pathogen.
- Understanding H pylori's interaction with host cells is crucial for developing effective treatments.
- Mouse-adapted strains are often used as models to study H pylori pathogenesis.
Purpose of the Study:
- To evaluate the in vitro adhesion and invasion capabilities of different mouse-adapted H pylori strains.
- To investigate the role of virulence factors CagA and VacA in H pylori's interaction with various cell lines.
- To examine the impact of bacterial strains and host cell types on H pylori adherence and invasion.
Main Methods:
- Gentamicin protection assay was employed to quantify H pylori adherence and invasion in epithelial cell lines.
- Null mutants for cagA and vacA were generated using direct PCR mutation.
- Microscopy was used to observe morphological changes in cell lines post-H pylori attachment.
Main Results:
- Adhesion and invasion efficiencies varied between H pylori strains and cell lines, differing from in vivo mouse models.
- The 88-3887 strain exhibited superior adhesion and invasion compared to SS1 and X47 strains.
- SCG-7901 cells demonstrated higher susceptibility to adhesion and invasion by all tested H pylori strains.
- CagA and VacA null mutants showed similar invasion and adhesion abilities to their wild-type counterparts.
- The hummingbird phenotype was observed only in AGS cells infected with the 88-3887 wild-type strain.
Conclusions:
- Both host cell type and bacterial strain characteristics significantly influence H pylori adhesion and invasion.
- The virulence factors CagA and VacA are not essential for H pylori's invasion and adhesion capabilities in vitro across different cell lines.
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