Polymorphonuclear leukocytes increase the adhesion of circulating tumor cells to microvascular endothelium
Miranda Ten Kate1, Arend G J Aalbers, Wim Sluiter
1Department of Surgery, Erasmus MC Rotterdam, The Netherlands.
Background:
Reactive oxygen species (ROS) released from activated polymorphonuclear leukocytes (PMN) during surgery may play a crucial role in the enhanced distant tumor recurrence after surgical trauma.
Materials And Methods:
The effect of PMN on the adhesion of the human colon carcinoma cells HT29, Caco2 and the pancreatic carcinoma cells PanC1 and BxPC3 to microvascular endothelium (MEC) was studied in a reproducible human in vitro model.
Results:
Pre-incubation of MEC with tissue plasminogen activator (TPA)-activated PMN resulted in more than 200% increase of tumor cell adhesion to MEC compared to control (p < 0.01). Exposure of MEC to TPA or non-activated PMN did not significantly affect adhesion. Addition of the antioxidant enzymes superoxide dismutase or catalase significantly decreased tumor cell adhesion to MEC exposed to PMN.
Conclusion:
These results demonstrate that activated PMN promote tumor cell adhesion to the microvascular wall by production of ROS. This indicates that in tackling the ROS production, preventing tumor recurrence at distant sites, might be feasible.
Related Concept Videos
Metastasis
Epithelial-to-Mesenchymal Transition
The epithelial-to-mesenchymal transition or EMT is a developmental process commonly observed in wound healing, embryogenesis, and cancer metastasis. EMT is induced by transforming growth factor-beta (TGF-β) or receptor tyrosine kinase (RTK) ligands, which further...
Selectins
The Tumor Microenvironment
Adaptive Mechanisms in Cancer Cells
Some of the advantages that cancer cells have on normal cells include - enhanced ability to divide without terminally differentiating, induce new blood vessel formation,...


