Related Experiment Video
Updated: Jul 16, 2026

Transfer of Manipulated Tumor-associated Neutrophils into Tumor-Bearing Mice to Study their Angiogenic Potential In Vivo
Published on: July 20, 2019
Neutrophil-activating protein-2- and interleukin-8-mediated angiogenesis
John A Powell1, Shaker A Mousa
1University of Pennsylvania, Medical School, Philadelphia, Pennsylvania, USA.
Nitric oxide (NO) donors and anti-integrin compounds inhibit angiogenesis induced by neutrophil-activating protein-2 (NAP-2) and basic fibroblast growth factor (FGF-2). These findings suggest potential therapeutic strategies for angiogenesis-related diseases.
Area of Science:
- Vascular Biology
- Pharmacology
Background:
- Angiogenesis, the formation of new blood vessels, is crucial for tumor growth and various diseases.
- Neutrophil-activating protein-2 (NAP-2), interleukin-8 (IL-8), and basic fibroblast growth factor (FGF-2) are key regulators of angiogenesis.
Purpose of the Study:
- To investigate the anti-angiogenic potential of nitric oxide (NO) donors and anti-integrin compounds.
- To evaluate their efficacy against NAP-2, IL-8, and FGF-2-induced angiogenesis.
Main Methods:
- Utilized the chick embryo chorioallantoic membrane (CAM) model for in vivo angiogenesis studies.
- Administered NO donors (e.g., S-nitroso N-acetyl penicillamine - SNAP) and anti-integrin agents targeting alphavbeta3 integrin.
Main Results:
- Both NO donors and anti-integrin agents effectively inhibited NAP-2 and FGF-2-induced angiogenesis in the CAM model.
- Anti-integrin compounds showed less inhibition against NAP-2 and IL-8 compared to FGF-2.
- Inhibition of endothelial cell differentiation was also observed.
Conclusions:
- Multiple pathways are involved in chemokine- and cytokine-mediated angiogenesis.
- NO donors and anti-integrin agents represent potential therapeutic strategies for inhibiting angiogenesis in human tumors and other neovascular diseases.
More Related Videos
14:04Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents
Published on: March 25, 2016
11:22Quantitative In vitro Assay to Measure Neutrophil Adhesion to Activated Primary Human Microvascular Endothelial Cells under Static Conditions
Published on: August 23, 2013
Related Concept Videos
Regulation of Angiogenesis and Blood Supply
Mechanism of Angiogenesis
Acute Inflammation II: Cellular Phase
Inflammation
Nitric Oxide Signaling Pathway
NF-κB-dependent Signaling Pathway
NF-κB-dependent Signaling Mechanism
The heterodimer of NF-κB...