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Updated: Jul 16, 2026
08:03
Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
Strain collections and genetic nomenclature
Stanley R Maloy1, Kelly T Hughes
1Department of Biology, Center for Microbial Sciences, San Diego State University, San Diego, CA, USA.
Methods in Enzymology
|March 14, 2007
Summary
Properly cataloging microbial mutants is essential for research accuracy. Assigning unique strain numbers and gene/allele names ensures clear identification and traceability of each mutant in laboratory collections.
Area of Science:
- Microbiology
- Genetics
- Molecular Biology
Background:
- Rapid accumulation of microbial mutants necessitates robust organizational systems.
- Confusion between similar mutants can hinder experimental reproducibility and data interpretation.
Purpose of the Study:
- To outline a standardized system for naming and numbering microbial mutants.
- To emphasize the importance of meticulous record-keeping in genetic research.
Main Methods:
- Assigning unique strain numbers (lab designation + serial number).
- Naming mutations by gene or phenotype and assigning allele numbers.
- Utilizing genetic stock centers for standardized nomenclature.
Main Results:
- A clear system for mutant identification prevents confusion.
- Standardized naming facilitates data sharing and collaboration.
- Accurate strain construction details (parental strain, DNA source) are crucial.
Conclusions:
- Implementing a systematic approach to mutant cataloging is vital for scientific integrity.
- Leveraging genetic stock centers ensures adherence to established nomenclature standards.
- Detailed strain information enhances the reliability and reproducibility of microbial genetics studies.
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