Jove
Visualize
Contact Us

Related Experiment Videos

Sets of transposon-generated sequence-tagged mutants for structure-function analysis and engineering.

Beth Traxler1, Eliora Gachelet

  • 1Department of Microbiology, University of Washington, Seattle, WA, USA.

Methods in Enzymology
|March 14, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression of soluble Type IV Minor Pilins and isolation of a <i>Neisseria gonorrhoeae</i> PilI-PilJ subcomplex.

bioRxiv : the preprint server for biology·2026
Same author

β-strand complementation within tip initiation complexes licenses assembly of diverse type IV filaments.

bioRxiv : the preprint server for biology·2026
Same author

Collaboration between a temperate phage and <i>Pseudomonas aeruginosa</i> quorum sensing constrains social cheats.

mBio·2025
Same author

Our friend and colleague Laura Frost: A brief tribute.

Plasmid·2025
Same author

Tradeoff between lag time and growth rate drives the plasmid acquisition cost.

Nature communications·2023
Same author

Editorial.

Plasmid·2019
Same journal

Clinical Europium fluorescent based lectin assays for mucin O-glycomics.

Methods in enzymology·2026
Same journal

A dual-color FRET assay for detection and quantitative analysis of O-glycopeptidases.

Methods in enzymology·2026
Same journal

Evolutionary genetic approaches to analyze mucins.

Methods in enzymology·2026
Same journal

Ex vivo imaging and enzymatic analysis of intestinal mucus.

Methods in enzymology·2026
Same journal

Glyco-TRAPP: A real-time glycocalyx permeability assay for assessing transmembrane mucin barrier function in live and fixed tissues.

Methods in enzymology·2026
Same journal

Quantitative imaging approaches to capture structural and functional dynamics of colonic mucus in health and disease in situ.

Methods in enzymology·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Researchers explore genetic strategies for creating insertional mutants. These mutant libraries aid in analyzing protein structure-function and advancing engineering applications.

Area of Science:

  • Genetics
  • Molecular Biology
  • Biotechnology

Background:

  • Insertional mutagenesis is a key technique in genetic research.
  • Understanding protein structure-function relationships is crucial for biological insights and applications.

Purpose of the Study:

  • To summarize genetic strategies for isolating small, in-frame insertional mutants.
  • To highlight the applications of resulting mutant libraries in protein analysis and engineering.

Main Methods:

  • Review of various genetic strategies for mutant library creation.
  • Analysis of published studies utilizing these mutant libraries.

Main Results:

  • Multiple genetic methods exist for generating targeted insertional mutants.

Related Experiment Videos

  • Mutant libraries are valuable tools for dissecting protein structure-function.
  • Applications extend to protein engineering and biotechnological advancements.

    • Conclusions:

    • Genetic strategies for creating insertional mutants are diverse and effective.
    • The application of mutant libraries significantly contributes to understanding protein behavior and developing new technologies.