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Related Experiment Video

Updated: Jul 16, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda

Following Cell-fate in E. coli After Infection by Phage Lambda

Published on: October 14, 2011

Probing nucleoid structure in bacteria using phage lambda integrase-mediated chromosome rearrangements.

Nathalie Garcia-Russell¹, Samantha S Orchard, Anca M Segall

¹Department of Biology, San Diego State University, San Diego, CA, USA.

Methods in Enzymology

|March 14, 2007

Summary

Conservative site-specific recombination is a versatile tool used in genetic engineering and biotechnology. This article highlights its application in studying chromosome structure and organization, alongside other genetic uses.

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Last Updated: Jul 16, 2026

Following Cell-fate in E. coli After Infection by Phage Lambda

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Site-specific Bacterial Chromosome Engineering: ΦC31 Integrase Mediated Cassette Exchange (IMCE)

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Published on: March 16, 2012

Area of Science:

Molecular Biology
Genetics
Biotechnology

Background:

Conservative site-specific recombination (CSSR) is a fundamental biological process with broad applications.
CSSR has been engineered for diverse uses in prokaryotic and eukaryotic systems.

Purpose of the Study:

To illustrate the utility of CSSR as a probe for chromosome structure and organization.
To provide a concise overview of other genetic engineering and biotechnology applications of CSSR.

Main Methods:

The study focuses on a specific example of CSSR application in chromosome analysis.
A summary of various other CSSR applications in genetics and biotechnology is presented.

Main Results:

Demonstrates the effectiveness of CSSR in investigating chromosome architecture.
Highlights the adaptability of CSSR for numerous genetic and biotechnological purposes.

Conclusions:

CSSR is a powerful and adaptable technology with significant implications in molecular biology and genetics.
The diverse applications of CSSR underscore its importance in scientific research and biotechnology development.