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Agrobacterium-Mediated Immature Embryo Transformation of Recalcitrant Maize Inbred Lines Using Morphogenic Genes

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GM maize from site-specific recombination technology, what next?

¹Plant Gene Expression Center, USDA-ARS and University of California at Berkeley, 800 Buchanan Street, Albany, California 94710, USA. david_ow@berkeley.edu

Current Opinion in Biotechnology

|March 14, 2007

Summary

Plant genetic engineering uses precise DNA manipulation for genome rearrangements. A new crop plant, utilizing this technology to remove markers, is nearing market, signaling broader applications.

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Area of Science:

Plant biotechnology
Molecular biology
Genomics

Background:

Plant genetic engineering offers precise genome manipulation.
Recombinase-based applications have advanced plant genome rearrangement techniques over two decades.
These techniques have evolved from laboratory exercises to practical applications.

Purpose of the Study:

To highlight the advancement of recombinase-based plant genome engineering.
To introduce the first crop plant developed using these advanced DNA manipulation techniques.
To suggest the potential for wider applications beyond marker removal.

Main Methods:

Utilizing recombinase-based systems for targeted DNA manipulation.
Applying these methods for efficient genome rearrangements in crop plants.
Developing a specific application for selectable marker removal.

Main Results:

A crop plant utilizing recombinase-based genome rearrangement is nearing market introduction.
The technology has proven effective for precise applications like marker removal.
This represents a significant step from laboratory research to commercial application.

Conclusions:

Recombinase-based plant genome engineering has reached a significant milestone with a commercial crop.
The technology's precision and efficiency pave the way for diverse future applications in plant breeding.
This development underscores the transition of advanced molecular tools into agricultural practice.