Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Pathway engineering by designed divergent evolution.

Yasuo Yoshikuni1, Jay D Keasling

  • 1UCSF/UCB Joint Graduate Group in Bioengineering, University of California at Berkeley, Berkeley, California 94720 USA.

Current Opinion in Chemical Biology
|March 14, 2007
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A biosynthetic survey of hypocrealean biocontrol fungi.

Nature chemical biology·2026
Same author

Expanding the scope of redox-balance growth coupling techniques with a carbon cofeeding strategy.

bioRxiv : the preprint server for biology·2026
Same author

Leveraging a synthetic biology approach to enhance BCG-mediated expansion of Vγ9Vδ2 T cells.

PloS one·2026
Same author

Quantitative Dissection of Agrobacterium Virulence to Generate a Synthetic Ti Plasmid.

ACS synthetic biology·2026
Same author

Synthetic biology for heterologous expression and engineering of fungal polyketide synthases.

Natural product reports·2026
Same author

Enzymology and Structural Basis of Glycosyltransferases Involved in Saponin C28 Carboxylic Acid <i>O</i>‑d‑Fucosylation.

JACS Au·2025
Same journal

Function through shape: An overview of DNA G-quadruplexes in transcriptional regulation.

Current opinion in chemical biology·2026
Same journal

Advances in tools and technologies for multiplexed bioluminescence imaging.

Current opinion in chemical biology·2026
Same journal

High-resolution molecular mapping by expansion-coupled label-free and multimodal imaging.

Current opinion in chemical biology·2026
Same journal

Recent advances in glycoconjugate-based therapeutics.

Current opinion in chemical biology·2026
Same journal

Towards better red emitters for bioimaging: Innovations in rhodamine and cyanine chemistry.

Current opinion in chemical biology·2026
Same journal

Chemigenetic fluorescent biosensors in biological imaging - New trends and advances.

Current opinion in chemical biology·2026
See all related articles

Protein engineering uses designed divergent evolution to create novel enzyme functions. This method predicts optimal mutations for specialized enzyme activity, enabling new applications in pharmaceuticals and agrochemicals.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Protein Engineering

Background:

  • Enzyme function can be redesigned using protein engineering methodologies.
  • Divergent molecular evolution theories suggest enzymes evolve from promiscuous to specialized functions via mutations.
  • The quasi-additive assumption posits that the effects of multiple mutations can be additive.

Purpose of the Study:

  • To introduce and validate a protein engineering methodology called designed divergent evolution.
  • To leverage theories of divergent molecular evolution for enzyme function redesign.
  • To enable the production of valuable natural and unnatural products.

Main Methods:

  • Utilizing theories of divergent molecular evolution, including plasticity and the quasi-additive assumption.

Related Experiment Videos

  • Estimating enzyme fitness landscapes by calculating the effects of single amino acid substitutions.
  • Predicting and selecting combinations of mutations for optimal enzyme function.

    • Main Results:

    • Designed divergent evolution is a powerful methodology for redesigning enzyme function.
    • The method allows for the estimation of enzyme fitness landscapes.
    • Predicted optimal mutations can be introduced into enzymes to achieve desired functions.

    Conclusions:

    • Designed divergent evolution effectively redesigns enzyme functions.
    • This methodology facilitates the creation of enzymes with enhanced or novel activities.
    • Redesigned enzymes can be utilized in metabolic pathways for producing pharmaceuticals, agrochemicals, and other products.