Related Experiment Video
Updated: Jul 16, 2026

Associated Chromosome Trap for Identifying Long-range DNA Interactions
Published on: April 23, 2011
Chromosomal 20q gain in the DNA diploid component of aneuploid colorectal carcinomas
Paula M De Angelis1, Trond Stokke, Marzieh Beigi
1The Pathology Clinic, University of Oslo, Rikshospitalet-Radiumhospitalet Medical Center, 0027 Oslo, Norway. p.d.angelis@medisin.uio.no
Abstract:
The order of appearance of different genetic aberrations during the shift from diploidy/near-diploidy to aneuploidy in colorectal cancers is not yet clear. We studied genetic alterations in flow cytometrically-sorted DNA diploid and corresponding aneuploid epithelial cell populations from each of 20 colorectal tumors using comparative genomic hybridization, FISH, and PCR. Analysis of the 19 cases in which aberrations were found in the flow-sorted diploid population indicated that large-scale aneuploidization in colorectal cancer was preceded by amplification of oncogene(s) localized to chromosome 20q13.2 and by KRAS mutations, but not by TP53 deletions or losses of large chromosomal regions such as 4q, 8p and 18q.
Related Concept Videos
Karyotyping
Karyotyping
Cancers Originate from Somatic Mutations in a Single Cell
Cancers Originate from Somatic Mutations in a Single Cell
Chromosome Duplication
The basic unit of the chromatin is the nucleosome, consisting of DNA wrapped around octameric histone proteins and short stretches of linker DNA separating individual nucleosomes. The histone proteins within the nucleosome have their...
Tumor Progression
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...

