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Updated: Aug 12, 2026
Mass Spectrometric Analysis of Glycosphingolipid Antigens
Published on: April 16, 2013
Extended type-1 chain glycosphingolipid antigens. Isolation and characterization of trifucosyl-Leb antigen
M R Stroud1, S B Levery, M E Salyan
1Biomembrane Institute, Seattle, WA 98119.
Abstract:
A Lewis-b-active glycosphingolipid containing a repetitive type-1 chain carbohydrate core was isolated from human colonic adenocarcinoma cell line Colo205. This glycosphingolipid was purified by HPLC and preparative high-performance thin-layer chromatography and its structure elucidated by positive-ion fast-atom-bombardment mass spectrometry with collision-induced disassociation, 1H-NMR spectroscopy and methylation analysis. The glycosphingolipid was found to be a trifucosylated derivative of this novel carbohydrate core, having the following structure: [formula; see text].
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