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Internalization efficiency of the transferrin receptor.

S H Hansen¹, K Sandvig, B van Deurs

¹Department of Anatomy, Panum Institute, University of Copenhagen, Denmark.

Experimental Cell Research

|March 1, 1992

Summary

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The transferrin receptor (TfR) is concentrated in coated pits, enhancing its internalization efficiency. This process is cell density-dependent, with higher densities leading to more efficient TfR uptake.

Area of Science:

Cell biology
Biochemistry
Membrane trafficking

Background:

The transferrin receptor (TfR) plays a crucial role in cellular iron uptake.
Understanding TfR internalization mechanisms is key to cellular homeostasis.

Purpose of the Study:

To quantitatively assess the internalization efficiency of the transferrin receptor (TfR).
To investigate the role of coated pits in TfR trafficking.
To determine the impact of cell density on TfR internalization.

Main Methods:

Quantitative ultrastructural analysis
Biochemical assays
Cell culture techniques at varying densities

Main Results:

Related Experiment Videos

Initial TfR internalization rate is ~10% per minute in confluent cultures.

Approximately 10% of cell-surface TfRs reside in coated pits, with a coated pit lifetime of ~1 minute.

TfRs are 6-9 fold concentrated in coated pits compared to other plasma membrane proteins.

TfR concentration in coated pits and internalization efficiency are cell density-dependent, increasing with higher cell density.

Conclusions:

Coated pits are critical for efficient TfR internalization.
Cell density significantly modulates TfR localization within coated pits and subsequent internalization rates.
These findings provide quantitative insights into receptor-mediated endocytosis regulation.